Enhancement of the killing effect of low-temperature plasma on Streptococcus mutans by combined treatment with gold nanoparticles

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• 作者：Sang Rye Park (1)
  Hyun Wook Lee (2)
  Jin Woo Hong (3)
  Hae June Lee (4)
  Ji Young Kim (1)
  Byul bo-ra Choi (5)
  Gyoo Cheon Kim (5)
  Young Chan Jeon (6)
  
  1. Department of Dental Hygiene ; Kyungnam College of Information and Technology ; Busan ; 617-701 ; Rep. Korea
  2. Department of Electrical Engineering ; Pohang University of Science and Technology ; Pohang ; 790-784 ; Rep. Korea
  3. Department of Korean Internal Medicine ; School of Korean Medicine ; Pusan National University ; Yangsan ; 626-870 ; Korea
  4. Department of Electronics Engineering ; Pusan National University ; Busan ; 609-735 ; Rep. Korea
  5. Department of Oral Anatomy ; School of Dentistry ; Pusan National University ; Yangsan ; 602-739 ; Rep. Korea
  6. Department of Dental Prosthetics ; School of Dentistry ; Pusan National University ; Yangsan ; 602-739 ; Republic of Korea
  
• 关键词：Gold nanoparticle ; Low ; temperature plasma ; Streptococcus mutans ; Sterilization ; Oral care
• 刊名：Journal of Nanobiotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：12
• 期：1
• 全文大小：977 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Nanotechnology
  
• 出版者：BioMed Central
• ISSN：1477-3155

文摘

Background Recently, non-thermal atmospheric pressure plasma sources have been used for biomedical applications such as sterilization, cancer treatment, blood coagulation, and wound healing. Gold nanoparticles (gNPs) have unique optical properties and are useful for biomedical applications. Although low-temperature plasma has been shown to be effective in killing oral bacteria on agar plates, its bactericidal effect is negligible on the tooth surface. Therefore, we used 30-nm gNPs to enhance the killing effect of low-temperature plasma on human teeth. Results We tested the sterilizing effect of low-temperature plasma on Streptococcus mutans (S. mutans) strains. The survival rate was assessed by bacterial viability stains and colony-forming unit counts. Low-temperature plasma treatment alone was effective in killing S. mutans on slide glasses, as shown by the 5-log decrease in viability. However, plasma treatment of bacteria spotted onto tooth surface exhibited a 3-log reduction in viability. After gNPs were added to S. mutans, plasma treatment caused a 5-log reduction in viability, while gNPs alone did not show any bactericidal effect. The morphological changes in S. mutans caused by plasma treatment were examined by transmission electron microscopy, which showed that plasma treatment only perforated the cell walls, while the combination treatment with plasma and gold nanoparticles caused significant cell rupture, causing loss of intracellular components from many cells. Conclusions This study demonstrates that low-temperature plasma treatment is effective in killing S. mutans and that its killing effect is further enhanced when used in combination with gNPs.