Application of ionic liquids based on imidazole to the electrophoretic determination of amino acids in urine

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• 作者：E. A. Kolobova ; L. A. Kartsova ; E. A. Bessonova
• 关键词：ionic liquids ; capillary zone electrophoresis ; micellar electrokinetic chromatography ; pseudostationary phase ; liquid–liquid extraction ; amino acids
• 刊名：Journal of Analytical Chemistry
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：70
• 期：11
• 页码：1354-1359
• 全文大小：287 KB
• 参考文献：1.Sun, P. and Armstrong, D.W., Anal. Chim. Acta, 2010, vol. 661, p. 1.CrossRef
  2.Krossing, I., Slattery, J.M., Daguenet, C., Dyson, P.J., Oleinikova, A., and Weingartner, H., J. Am. Chem. Soc., 2006, vol. 128, nos. 41, p. 13427.CrossRef
  3.Huddleston, J.G., Visser, A.E., Reichert, W.M., Willauer, H.D., Broker, G.A., and Rogers, R.D., Green Chem., 2001, vol. 3, p. 156.CrossRef
  4.He, L., Zhang, W., Zhao, L., Liu, X., and Jiang, S., J. Chromatogr. A, 2003, vol. 1007, p. 39.CrossRef
  5.Zhang, W., He, L., Gu, Y., Liu, X., and Jiang, S., Anal. Lett., 2003, vol. 36, p. 827.CrossRef
  6.Yanes, E.G., Gratz, S.R., Baldwin, M.J., Robison, S.E., and Stalcup, A.M., Anal. Chem., 2001, vol. 73, p. 3838.CrossRef
  7.Niu, J., Qiu, H., Li, J., Liu, X., and Jiang, S., J. Chromatogr. A, 2009, vol. 69, p. 1093.CrossRef
  8.Absalan, G., Akhond, M., and Sheikhian, L., Talanta, 2008, vol. 77, p. 407.CrossRef
  9.Pei, Y., Wanga, J., Wua, K., Xuana, X., and Lub, X., Sep. Purif. Technol., 2009, vol. 64, p. 288.CrossRef
  10.Vidal, L., Parshintsev, J., Hartonen, K., Canals, A., and Riekkola, M.-L., J. Chromatogr. A, 2012, vol. 1226, p. 2.CrossRef
  11.Jungnickel, C., Luczak, J., Ranke, J., Fernandez, J., Muller, A., and Thoming, J., Colloids Surf., A, 2008, vol. 316, p. 278.CrossRef
  12.Smirnova, S.V., Torocheshnikova, I.I., Formanovsky, A.A., and Pletnev, I.V., Anal. Bioanal. Chem., 2004, vol. 378, p. 1369.CrossRef
  13.Wang, J., Pei, Y., Zhao, Y., and Hu, Z., Green Chem., 2005, vol. 7, p. 196.CrossRef
  
• 作者单位：E. A. Kolobova (1)
  L. A. Kartsova (1)
  E. A. Bessonova (1)
  
  1. Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, St. Petersburg, 198504, Russia
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3199

文摘

The effects of the ionic liquids C₁₂MImCl and C₁₆MImCl as background electrolyte components on the migration parameters of amino acids under the conditions of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were found. It was established that the introduction of the above ionic liquids based on imidazole into a background electrolyte (phosphate buffer solution, pH 2.0) led to an increase in efficiency by a factor of 2- in the determination of tryptophan, tyrosine, and 3,4-dihydroxyphenylalanine under the conditions of CZE and in separation selectivity in the MEKC mode. The application of intracapillary concentration (field-amplified sample stacking) with the use of C₁₆MImCl as a background electrolyte constituent made it possible to concentrate tryptophan, tyrosine, and 3,4-dihydroxyphenylalanine by a factor of 10-4. The limits of detection of the amino acids were 30-5 ng/mL. It was found that the inclusion of C₁₂MImCl into the composition of the electrophoretic system makes it possible to determine glycine, which does not absorb in the UV of the region of the spectrum, under the conditions of indirect detection at 220 nm. A procedure was proposed for urine sample preparation for the electrophoretic determination of amino acids with the use of C₆MImNTf₂ as an extractant. The degrees of amino acid recovery were 92-00%. Keywords ionic liquids capillary zone electrophoresis micellar electrokinetic chromatography pseudostationary phase liquid–liquid extraction amino acids