Study of metabolic network of Cupriavidus necator DSM 545 growing on glycerol by applying elementary flux modes and yield space analysis
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- 作者：Markan Lopar (1)
Ivna Vrana ?poljari? (1)
Nikolina Cepanec (1)
Martin Koller (2) (3) (4)
Gerhart Braunegg (3)
Predrag Horvat (1) - 关键词：Cupriavidus necator ; Elementary flux modes ; Glycerol ; PHB ; Yield space analysis
- 刊名：Journal of Industrial Microbiology and Biotechnology
- 出版年：2014
- 出版时间：June 2014
- 年：2014
- 卷：41
- 期：6
- 页码：913-930
- 全文大小：
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Ivna Vrana ?poljari? (1)
Nikolina Cepanec (1)
Martin Koller (2) (3) (4)
Gerhart Braunegg (3)
Predrag Horvat (1)
1. Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6/IV, 10000, Zagreb, Croatia
2. Institute of Biotechnology and Biochemical Engineering, Graz University of Technology, Petersgasse 12, 8010, Graz, Austria
3. ARENA, Arbeitsgemeinschaft für Ressourcenschonende and Nachhaltige Technologien, Graz University of Technology, Inffeldgasse 23, 8010, Graz, Austria
4. Institute of Chemistry, University of Graz, Stremayrgasse 16/IV, 8010, Graz, Austria - ISSN：1476-5535
文摘
A metabolic network consisting of 48 reactions was established to describe intracellular processes during growth and poly-3-hydroxybutyrate (PHB) production for Cupriavidus necator DSM 545. Glycerol acted as the sole carbon source during exponential, steady-state cultivation conditions. Elementary flux modes were obtained by the program Metatool and analyzed by using yield space analysis. Four sets of elementary modes were obtained, depending on whether the pair NAD/NADH or FAD/FADH2 contributes to the reaction of glycerol-3-phosphate dehydrogenase (GLY-3-P DH), and whether 6-phosphogluconate dehydrogenase (6-PG DH) is present or not. Established metabolic network and the related system of equations provide multiple solutions for the simultaneous synthesis of PHB and biomass; this number of solutions can be further increased if NAD/NADH or FAD/FADH2 were assumed to contribute in the reaction of GLY-3-P DH. As a major outcome, it was demonstrated that experimentally determined yields for biomass and PHB with respect to glycerol fit well to the values obtained in silico when the Entner–Doudoroff pathway (ED) dominates over the glycolytic pathway; this is also the case if the Embden–Meyerhof–Parnas pathway dominates over the ED.