Isolation and characterization of a Pseudomonas aeruginosa from a virgin Brazilian Amazon region with potential to degrade atrazine
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- 作者：Ana Flavia Tonelli Fernandes (1)
Michelle Barbosa Partata da Silva (1)
Vinicius Vicente Martins (1)
Carlos Eduardo Saraiva Miranda (2)
Eliana Guedes Stehling (1) - 关键词：Pseudomonas aeruginosa ; Atrazine ; Brazilian Amazon Forest
- 刊名：Environmental Science and Pollution Research
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：21
- 期：24
- 页码：13974-13978
- 全文大小：278 KB
- 参考文献：1. Bohn T, Cocco E, Gourdol L, Guignard C, Hoffmann L (2011) Determination of atrazine and degradation products in Luxembourgish drinking water: origin and fate of potential endocrine-disrupting pesticides. Food Addit Contam Part A 28:1041-054 CrossRef
2. de Souza ML, Wackett LP, Sadowsky MJ (1998a) The atzABC genes encoding atrazine catabolism genes are located on a self-transmissible plasmid in / Pseudomonas strain ADP. Appl Environ Microbiol 64:2323-326
3. de Souza M, Seffernick J, Martinez B, Sadowsky MJ, Wackett LP (1998b) The atrazine catabolism genes / atzABC are widespread and highly conserved. J Bacteriol 180:1951-954
4. de Souza ML, Newcombe D, Alvey S, Crowley DE, Hay A, Sadowsky MJ, Wackett LP (1998c) Molecular basis of a bacterial consortium: interspecies catabolism of atrazine. Appl Environ Microbiol 64:178-84
5. De Vos D, Lim A Jr, Pirnay JP, Duinslaeger L, Revets H, Vanderkelen A, Hamers R, Cornelis P (1997) Analysis of epidemic / Pseudomonas aeruginosa isolates by isoeletric focusing of pyoverdine and RAPD-PCR: modern tools for an integrated anti-nosocomial infection strategy in burn wound centers. Burns 23:379-86 CrossRef
6. Devers M, Soulas G, Martin-Laurent F (2004) Real-time reverse transcription PCR analysis of expression of atrazine catabolism genes in two bacterial strains isolated from soil. J Microbiol Methods 56:3-5 CrossRef
7. Eaton RW, Karns JS (1991) Cloning and comparison of the DNA encoding ammelide aminohydrolase and cyanuric acid amidohydrolase from three / s-triazine-degrading bacterial strains. J Bacteriol 173:1363-366
8. Gao Y, Fang J, Zhang J, Ren L, Mao Y, Li B et al (2011) The impact of the herbicide atrazine on growth and photosynthesis of seagrass, / Zostera marina (L.), seedlings. Mar Pollut Bull 62:1628-631 CrossRef
9. Ghimire N, Woodward RT (2013) Under- and over-use of pesticides: an international analysis. Ecol Econ 89:73-1 CrossRef
10. Hernández M, Villalobos P, Morgante V, González M, Reiff C, Moore E et al (2008) Isolation and characterization of a novel simazine-degrading bacterium from agricultural soil of central Chile, / Pseudomonas sp. MHP41. FEMS Microbiol Lett 286:184-90 CrossRef
11. Karns JS (1999) Gene sequence and properties of an / s-triazine ring-cleavage enzyme from / Pseudomonas sp. strain NRRLB-12227. Appl Environ Microbiol 65:3512-517
12. Macrina FL, Kopecko DJ, Jones KR, Ayers DJ, McCowen SM (1987) A multiple plasmid-containing / E. coli strain: convenient source of size reference plasmid molecules. Plasmid 1:417-20 CrossRef
13. Mandelbaum RT, Allan DL, Wackett LP (1995) Isolation and characterization of a / Pseudomonas aeruginosa sp. that mineralizes the s-triazine herbicide atrazine. Appl Environ Microbiol 61:1451-457
14. Migeot V, Albouy-Llaty M, Carles C, Limousi F, Strezlec S, Dupuis A et al (2013) Drinking-water exposure to a mixture of nitrate and low-dose atrazine metabolites and small-for-gestational age (SGA) babies: a historic cohort study. Environ Res 122:58-4 CrossRef
15. Mukherjee K, Tribedi P, Chowdhury A, Ray T, Joardar A, Giri S et al (2011) Isolation of a / Pseudomonas aeruginosa strain from soil that can degrade polyurethane diol. Biodegradation 22:377-88 CrossRef
16. Mulbry WW, Zhu H, Nour SM, Topp E (2002) The triazine hydrolase gene / trzN from / Nocardioides sp. strain C190: cloning and construction of gene-specific primers. FEMS Microbiol Lett 206:75-9 CrossRef
17. Platero AI, García-Jaramillo M, Santero E, Govantes F (2012) Transcriptional organization and regulatory elements of a / Pseudomonas sp. strain ADP operon encoding a Lys-type regulator and a putative solute transport system. - 作者单位：Ana Flavia Tonelli Fernandes (1)
Michelle Barbosa Partata da Silva (1)
Vinicius Vicente Martins (1)
Carlos Eduardo Saraiva Miranda (2)
Eliana Guedes Stehling (1)
1. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto, USP-Ribeir?o Preto, Av. do Café S/N. Monte Alegre, Ribeir?o Preto, SP, Brazil, 14040-903
2. Curso de Ciências Farmacêuticas de Ribeir?o Preto, Universidade de Ribeir?o Preto (UNAERP), Ribeir?o Preto, Brazil - ISSN：1614-7499
文摘
The use of pesticides to increase agricultural production can result in the contamination of the environment, causing changes in the genetic structure of organisms and in the loss of biodiversity. This practice is also inducing changes in the rainforest ecosystem. In this work, a Pseudomonas aeruginosa isolated from a preservation soil area of the Brazilian Amazon Forest, without usage of any pesticide, was evaluated for its potential to degrade atrazine. This isolate presented all responsible genes (atzA, atzB, atzC, atzD, atzE, and atzF) for atrazine mineralization and demonstrated capacity to use atrazine as a nitrogen source, having achieved a reduction of 44?% of the initial concentration of atrazine after 24?h. These results confirm gene dispersion and/or a possible contamination of the area with the herbicide, which reinforces global concern of the increase and intensive use of pesticides worldwide.