Repetitive DNA Sequences in the Common Vole: Cloning, Characterization and Chromosome Localization of Two Novel Complex Repeats MS3 and MS4 from the Genome of the East European Vole Microtus Rossiaeme

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• 作者：Elisaphenko ; Eugene A. ; Nesterova ; Tatyana B. ; Duthie ; Sarah M. ; Ruldugina ; Olga V. ; Rogozin ; Igor B. ; Brockdorff ; Neil ; Zakian ; Suren M.
• 关键词：heterochromatin ; repeats ; sex chromosomes vole
• 刊名：Chromosome Research
• 出版年：1998
• 出版时间：1998
• 年：1998
• 卷：6
• 期：5
• 页码：351-360
• 全文大小：292 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Human Genetics
  Animal Genetics and Genomics
  Plant Genetics and Genomics
  
• 出版者：Springer Netherlands
• ISSN：1573-6849

文摘

We have characterized two novel, complex, heterochromatic repeat sequences, MS3 and MS4, isolated from Microtus rossiaemeridionalis genomic DNA. Sequence analysis indicates that both repeats consist of unique sequences interrupted by repeat elements of different origin and can be classified as long complex repeat units (LCRUs). A unique feature of both repeat units is the presence of short interspersed repeat elements (SINEs), which are usually characteristic of the euchromatic part of the genome. Comparative analysis revealed no significant stretches of homology in the nucleotide sequences between the two repeats, suggesting that the repeats originated independently during the course of vole genome evolution. Fluorescence in situ hybridization analysis demonstrates that MS3 and MS4 occupy distinct domains in the heterochromatic regions of the sex chromosomes in M. transcaspicus and M. arvalis but collocalize in M. rossiaemeridionalis and M. kirgisorum heterochromatic blocks. The localization pattern of the repeats on the vole chromosomes confirms the independent origin of the two repeats and suggests that expansion of the heterochromatic blocks has occurred subsequent to speciation.