Temporal dynamics of microbial communities in the rhizosphere of two genetically modified (GM) maize hybrids in tropical agrosystems
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- 作者：Simone Raposo Cotta (1)
Armando Cavalcante Franco Dias (2)
Ivanildo Evódio Marriel (3)
Eliane Aparecida Gomes (3)
Jan Dirk van Elsas (4)
Lucy Seldin (1) - 关键词：PCR ; DGGE ; Rhizosphere ; Transgenic crops ; Genetically modified (GM) maize
- 刊名：Antonie van Leeuwenhoek
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：103
- 期：3
- 页码：589-601
- 全文大小：780KB
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Armando Cavalcante Franco Dias (2)
Ivanildo Evódio Marriel (3)
Eliane Aparecida Gomes (3)
Jan Dirk van Elsas (4)
Lucy Seldin (1)
1. Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Bloco I Ilha do Fund?o, Rio de Janeiro, RJ, CEP 21941-590, Brazil
2. Departamento de Ciência do Solo, Escola Superior de Agricultura Luiz de Queiroz, Universidade de S?o Paulo, Piracicaba, SP, Brazil
3. EMBRAPA/CNPMS - Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisas de Milho e Sorgo, Sete Lagoas, MG, Brazil
4. Department of Microbial Ecology, Center for Ecological and Evolutionary Studies (CEES), University of Groningen (RUG), Nijenborgh 7, 9747 AG, Groningen, The Netherlands - ISSN：1572-9699
文摘
The use of genetically modified (GM) plants still raises concerns about their environmental impact. The present study aimed to evaluate the possible effects of GM maize, in comparison to the parental line, on the structure and abundance of microbial communities in the rhizosphere. Moreover, the effect of soil type was addressed. For this purpose, the bacterial and fungal communities associated with the rhizosphere of GM plants were compared by culture-independent methodologies to the near-isogenic parental line. Two different soils and three stages of plant development in two different periods of the year were included. As evidenced by principal components analysis (PCA) of the PCR-DGGE profiles of evaluated community, clear differences occurred in these rhizosphere communities between soils and the periods of the year that maize was cultivated. However, there were no discernible effects of the GM lines as compared to the parental line. For all microbial communities evaluated, soil type and the period of the year that the maize was cultivated were the main factors that influenced their structures. No differences were observed in the abundances of total bacteria between the rhizospheres of GM and parental plant lines.