Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall
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- 作者：Vincent Phalip ; Fran?ois Delalande ; Christine Carapito ; Florence Goubet ; Didier Hatsch ; Emmanuelle Leize-Wagner ; Paul Dupree ; Alain Van Dorsselaer and Jean-Marc Jeltsch
- 关键词：Fungi ; Fusarium graminearum ; Hydrolases ; Plant cell wall ; Polysaccharide degradation ; CWDE regulation
- 刊名：Current Genetics
- 出版年：2005
- 出版时间：December 2005
- 年：2005
- 卷：48
- 期：6
- 页码：366-379
- 全文大小：410 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbial Genetics and Genomics
Microbiology
Biochemistry
Cell Biology
Plant Sciences
Proteomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-0983
文摘
The exoproteome of the fungus Fusarium graminearum grown on glucose and on hop (Humulus lupulus, L.) cell wall has been investigated. The culture medium was found to contain a higher quantity of proteins and the proteins are more diverse when the fungus is grown on cell wall. Using both 1D and 2D electrophoresis followed by mass spectrometry analysis and protein identification based on similarity searches, 84 unique proteins were identified in the cell wall-grown fungal exoproteome. Many are putatively implicated in carbohydrate metabolism, mainly in cell wall polysaccharide degradation. The predicted carbohydrate-active enzymes fell into 24 different enzymes classes, and up to eight different proteins within a same class are secreted. This indicates that fungal metabolism becomes oriented towards synthesis and secretion of a whole arsenal of enzymes able to digest almost the complete plant cell wall. Cellobiohydrolase is one of the only four proteins found both after growth on glucose and on plant cell wall and we propose that this enzyme could act as a sensor of the extracellular environment. Extensive knowledge of this very diverse F. graminearum exoproteome is an important step towards the full understanding of Fusarium/plants interactions.