Development of curcumin-loaded poly(hydroxybutyrate-co-hydroxyvalerate) nanoparticles as anti-inflammatory carriers to human-activated endothelial cells

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• 作者：Viorel Simion (1)
  Daniela Stan (1)
  Ana-Maria Gan (1)
  Monica Madalina Pirvulescu (1)
  Elena Butoi (1)
  Ileana Manduteanu (1)
  Mariana Deleanu (1)
  Eugen Andrei (1)
  Anamaria Durdureanu-Angheluta (2)
  Marian Bota (3)
  Marius Enachescu (3)
  Manuela Calin (1) (2)
  Maya Simionescu (1)
  
• 关键词：Curcumin ; Endothelium ; Inflammation ; Polymeric nanoparticles ; Poly(hydroxybutyrate ; co ; hydroxyvalerate)
• 刊名：Journal of Nanoparticle Research
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：15
• 期：12
• 全文大小：
• 作者单位：Viorel Simion (1)
  Daniela Stan (1)
  Ana-Maria Gan (1)
  Monica Madalina Pirvulescu (1)
  Elena Butoi (1)
  Ileana Manduteanu (1)
  Mariana Deleanu (1)
  Eugen Andrei (1)
  Anamaria Durdureanu-Angheluta (2)
  Marian Bota (3)
  Marius Enachescu (3)
  Manuela Calin (1) (2)
  Maya Simionescu (1)
  
  1. Institute of Cellular Biology and Pathology icolae Simionescuof the Romanian Academy, 8, BP Hasdeu Street, PO Box 35-14, 050568, Bucharest, Romania
  2. Institute of Macromolecular Chemistry etru Poniof the Romanian Academy, Iasi, Romania
  3. Center for Surface Science and Nanotechnology, Politehnica University, Bucharest, Romania
  
• ISSN：1572-896X

文摘

Curcumin (Cm)-loaded poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) nanoparticles (CmPN) were obtained and characterized and their effect on human endothelial cells (HEC) was assessed. Different CmPN formulations have been prepared using the emulsion solvent evaporation technique, and characterized for size, structure, Zeta potential, Cm entrapment efficiency, and in vitro Cm release. CmPN cytotoxicity and cellular uptake have been followed using HEC. Also, the effect of CmPN treatment on the p38MAPK signaling pathway in endothelial cells was investigated. The results obtained by electron and atomic force microscopy revealed the spherical shape of the CmPN formulation. Based on size and encapsulation efficiency, the CmPN formulation with the average diameter of 186nm and with the highest encapsulation efficiency (83%) has been used in the further studies. The release of Cm from CmPN was ~18% after 8h of incubation at 37, followed by a slow release until 144h, when it reached 44%, indicating a controlled release. CmPN are taken up by HEC and exhibited low cytotoxicity at concentrations up to 10. The pre-treatment of HEC with CmPN before exposure to tumor necrosis factor-alpha (TNF- determined a decrease of p38MAPK phosphorylation. In conclusion, Cm encapsulated into PHBV nanoparticles, at concentration up to 10, has low cytotoxicity and display anti-inflammatory activity on TNF-activated HEC by suppressing the phosphorylation of p38MAPK.