Comparative study for the detection of antibodies to Neospora caninum in milk and sera in dairy cattle in southern Romania
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- 作者：Violeta Enachescu (1)
Mariana Ionita (1)
Ioan Liviu Mitrea (1) - 关键词：Cattle ; Neospora caninum ; IgG antibodies ; serum ; milk ; Romania
- 刊名：Acta Parasitologica
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：59
- 期：1
- 页码：5-10
- 全文大小：531 KB
- 参考文献：1. Altman D.G. 1991. Practical statistics for medical research. Chapman and Hall, London.
2. Bartels C.J.M., van Maanen C., van der Meulen A.M., Dijkstra T., Wouda W. 2005. Evaluation of three enzyme-linked immunosorbent assays for detection of antibodies to / Neospora caninum in bulk milk. / Veterinary Parasitology, 131, 235鈥?46. DOI: 10.1016/j.vetpar.2005.05.011. CrossRef
3. Bj枚rkman C., Johannsson O., Stenlund S., Holmdahl O.J.M., Uggla A. 1996. / Neospora species infection in a herd of dairy cattle. / Journal of the American Veterinary Medical Association, 208, 1441鈥?444.
4. Byrem T.M., Bartlett P.C., Donohue H., Voisinet B.D., Houseman J.T. 2012. Performance of a commercial serum ELISA for the detection of antibodies to / Neospora caninum in whole and skim milk samples. / Veterinary Parasitology, 190, 249鈥?53. DOI: 10.1016/j.vetpar.2012.06.005. CrossRef
5. Conraths J., Gottstein B. 2007. Neosporosis: General considerations. In: (Eds. L.M. Ortega-Mora, B. Gottstein, F.J. Conraths and D. Buxton) Protozoal abortion in farm ruminants. CAB International, Wallingford, 42鈥?6.
6. Dubey J.P., Schares G. 2006. Diagnosis of bovine neosporosis. / Veterinary Parasitology, 140, 1鈥?4. DOI: 10.1016/j.vetpar.2006.03.035. CrossRef
7. Dubey J.P., Schares G. 2011. Neosporosis in animals 鈥?The last five years. / Veterinary Parasitology, 180, 90鈥?09. DOI: 10.1016/j.vetpar.2011.05.031. CrossRef
8. Dubey J.P., Jenkins M.C., Rajendran C., Miska K., Ferreira L.R., Martins J., Kwok O.C., Choudhary S. 2011. Gray wolf ( / Canis lupus) is a natural definitive host for / Neospora caninum. / Veterinary Parasitology, 181, 382鈥?87. DOI: 10.1016/j.vetpar. 2011.05.018. CrossRef
9. Frossling J., Lindberg A., Bjorkman C. 2006. Evaluation of an iscom ELISA used for detection of antibodies to / Neospora caninum in bulk milk. / Preventive Veterinary Medicine, 74, 120鈥?29. DOI: 10.1016/j.prevetmed.2005.11.012. CrossRef
10. Gavrea R.R., Cozma V. 2010. Seroprevalence of / Neospora caninum in cows with reproductive failure in Center and Northwest of Romania. / Sciencia Parasitologica, 11, 67鈥?0.
11. Gavrea R.R., Iovu A., Losson B., Cozma V. 2011. Seroprevalence of / Neospora caninum in dairy cattle from north-west and centre of Romania. / Parasite, 18, 349鈥?51. DOI: 10.1051/parasite/2011184349. CrossRef
12. Gondim L.F., McAllister M.M., Pitt W.C., Zemlicka D.E. 2004. Coyotes ( / Canis latrans) are definitive hosts of / Neospora caninum. / International journal for Parasitology, 34, 159鈥?61. CrossRef
13. Gonz谩lez-Warleta M., Castro-Hermida J.A., Carro-Corral C., Mezo M. 2011. Anti- / Neospora caninum antibodies in milk in relation to production losses in dairy cattle. / Preventive Veterinary Medicine, 101, 58鈥?4. DOI: 10.1016/j.prevetmed. 2011.04.019. CrossRef
14. Hall C.A., Reichel M.P., Ellis J.T. 2006. Prevalence of / Neospora caninum infection in Australian (NSW) dairy cattle estimated by a newly validated ELISA for milk. / Veterinary Parasitology, 142, 173鈥?78. DOI: 10.1016/j.vetpar.2006.06.019. CrossRef
15. Hemphill A., Gottstein B. 2006. / Neospora caninum and neosporosis 鈥?recent achievements in host and parasite cell biology and treatment. / Acta Parasitologica, 51, 15鈥?5. DOI:10.2478/s11686-006-0002-z. CrossRef
16. Hurley W.L., Theil P.K. 2011. Perspectives on Immunoglobulins in Colostrum and Milk. / Nutrients, 3, 442鈥?74. DOI: 10.3390/nu3040442. CrossRef
17. Imre K., Morariu S., Ilie M.S., Imre M., Ferrari N., Genchi C., D膬r膬bu艧 G. 2012. Serological survey of / Neospora caninum infection in cattle herds from Western Romania. / Journal of Parasitology, 98, 683鈥?85. DOI: 10.1645/GE-3023.1. CrossRef
18. Jenkins M.C., Wouda W., Dubey J.P. 1997. Serological response over time to recombinant / Neospora caninum antigens in cattle after a neosporosis-induced abortion. / Clinical and Diagnostic Laboratory Immunology, 4, 270鈥?74.
19. King J.S., Slapeta J., Jenkins D.J., Al-Qassab S.E., Ellis J.T., Windsor P.A. 2010. Australian dingoes are definitive hosts of / Neospora caninum. / International Journal for Parasitology, 40, 945鈥?50. DOI: 10.1016/j.ijpara.2010.01.008. CrossRef
20. McAllister M.M., Dubey J.P., Lindsay D.S., Jolley W.R., Wills R.A., McGuire A.M. 1998. Dogs are definitive hosts of / Neospora caninum. / International Journal for Parasitology, 28, 1473鈥?478. DOI: 10.1016/S0020-7519(98)00138-6. CrossRef
21. Mitrea I.L., Enachescu V., R膬dulescu R., Ionita M. 2012. Seroprevalence of / Neospora caninum infection on dairy cattle in farms from southern Romania. / Journal of Parasitology, 98, 69鈥?2. DOI: 10.1645/GE-2972.1. CrossRef
22. Moore D.P., P茅rez A., Agliano S., Brace M., Cant贸n G., Cano D., Leunda M.R., Ode贸n A.C., Odriozola E., Campero C.M. 2009. Risk factors associated with / Neospora caninum infections in cattle in Argentina. / Veterinary Parasitology, 161, 122鈥?25. DOI: 10.1016/j.vetpar.2009.01.003. CrossRef
23. Par茅 J., Thurmond M.C., Hietala S.K. 1997. / Neospora caninum antibodies in cows during pregnancy as a predictor of congenital infection and abortion. / Journal of Parasitology, 83, 82鈥?7. CrossRef
24. Schares G., Barwald A., Staubach C., Wurm R., Rauser M., Conraths F.J., Schroeder C. 2004. Adaptation of a commercial ELISA for the detection of antibodies against / Neospora caninum in bovine milk. / Veterinary Parasitology, 120, 55鈥?3. DOI: 10.1016/j.vetpar.2003.11.016. CrossRef
25. Wapenaar W., Barkema H.W., O鈥橦andley R.M., Bartels C.J. 2007. Use of an enzyme-linked immunosorbent assay in bulk milk to estimate the prevalence of / Neospora caninum on dairy farms in Prince Edward Island, Canada. / Canadian Veterinary Journal, 48, 493鈥?99.
26. Wu J.T.Y., Dreger S., Chow E.Y.W., Bowlby E.E. 2002. Validation of 2 commercial / Neospora caninum enzyme linked immunosorbent assays. / Canadian Journal for Veterinary Research, 66, 264鈥?71.
27. Zweig M.H., Campbell G. 1993. Receiver-operating characteristic (ROC) plots: a fundamental evaluation tool in clinical medicine. / Clinical Chemistry, 39, 561鈥?77. - 作者单位：Violeta Enachescu (1)
Mariana Ionita (1)
Ioan Liviu Mitrea (1)
1. Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine of Bucharest, University of Agronomic Sciences and Veterinary Medicine, 050097, Splaiul Independentei, 105, Bucharest, Romania - ISSN：1896-1851
文摘
The study aimed to assess the within-herd Neospora caninum exposure in dairy cattle in southern Romania, based on the detection of specific antibodies in milk and serum. A total of 104 paired samples of milk and serum were collected from four dairy farms. Individual samples were analyzed for N. caninum antibodies by ELISA: IDEXX Neospora Ab (Idx) (three farms: A, B, C; n = 60) and ID-VET Lab (Idv) (farm D; n = 44). Additionally, four pooled milk samples, one per each farm (A, B, C) and a composed one (A+B+C), were analyzed with Idx ELISA. Optimized cut-off values for milk samples were determined by receiver operating characteristic (ROC) analysis, with serum results considered as true status. The agreement was expressed by K values. The overall seroprevalence of N. caninum infection was 45% in the farms tested by Idx ELISA and 56.8% in the farm tested by Idv ELISA. A good agreement between serum and milk was obtained for both ELISA kits (K = 0.72 and 0.77, respectively). The specificity and sensitivity at optimized cut-off of S/P>0.704 for Idx and S/P%>7.966% for Idv were 100% and 70.37% for Idx and 89.47% and 88% for Idv. Testing pooled milk samples, there were identified as N. caninum positive the dairy farms with a 15% or higher within-herd seroprevalence at the cut-off value of S/P>0.51. This is the first study in Romania in which milk samples were tested to determine the N. caninum infection status in dairy farms, providing a base for further researches.