Prevalence and diversity of Encephalitozoon spp. and Enterocytozoon bieneusi in wild boars (Sus scrofa) in Central Europe

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• 作者：Karel Němejc (1)
  Bohumil Sak (2)
  Dana Květoňová (2)
  Vladimír Hanzal (1) (3)
  Pawe? Janiszewski (4)
  Pavel Forejtek (5)
  Du?an Rajsky (6)
  Michaela Kotková (2)
  Petra Ravaszová (7)
  John McEvoy (8)
  Martin Kvá? (1) (2)
  
• 刊名：Parasitology Research
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：113
• 期：2
• 页码：761-767
• 全文大小：366 KB
• 作者单位：Karel Němejc (1)
  Bohumil Sak (2)
  Dana Květoňová (2)
  Vladimír Hanzal (1) (3)
  Pawe? Janiszewski (4)
  Pavel Forejtek (5)
  Du?an Rajsky (6)
  Michaela Kotková (2)
  Petra Ravaszová (7)
  John McEvoy (8)
  Martin Kvá? (1) (2)
  
  1. Faculty of Agriculture, University of South Bohemia in ?eské Budějovice, ?eské Budějovice, Czech Republic
  2. Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, v.v.i., Brani?ovská 31, ?eské Budějovice, 370 05, Czech Republic
  3. Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Prague, Czech Republic
  4. Faculty of Animal Bioengineering, University of Warmia and Mazury, Olsztyn, Kortowo, Poland
  5. Institute for Wildlife Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic
  6. Faculty of Forestry, Technical University in Zvolen, Zvolen, Slovak Republic
  7. Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy in Ko?ice, Ko?ice, Slovak Republic
  8. Veterinary and Microbiological Sciences Department, North Dakota State University, Fargo, ND, USA
  
• ISSN：1432-1955

文摘

From 2011 to 2012, the occurrence of Enterocytozoon bieneusi and Encephalitozoon spp. was surveyed at 29 randomly selected localities (both forest areas and enclosures) across four Central European countries: Austria, the Czech Republic, Poland, and the Slovak Republic. Isolates were genotyped by PCR amplification and characterization of the internal transcribed spacer (ITS) region using Enterocytozoon and Encephalitozoon-specific protocols. PCR revealed 16 mono-infections of Encephalitozoon cuniculi, 33 mono-infections of Enterocytozoon bieneusi and 5 concurrent infections of both Encephalitozoon cuniculi and Enterocytozoon bieneusi out of 460 faecal samples. Two genotypes (I and II) were revealed by sequence analysis of the ITS region of Encephalitozoon cuniculi. Eleven genotypes, five previously found in other hosts including domestic pigs (D, EbpA, EbpC, G and Henan-I) and six novel (WildBoar1-), were identified in Enterocytozoon bieneusi. No other microsporidia infection was found in the examined faecal samples. Prevalence of microsporidia at the locality level ranged from 0 to 58.8?%; the prevalence was less than 25?% at more than 86?% of localities. Enterocytozoon bieneusi was detected as a predominant species infecting Eurasian wild boars (Sus scrofa). The present report is the most comprehensive survey of microsporidia infections in wild boars within the Czech Republic and selected Central European countries.