Inactivation of non-enveloped virus by 1,5 iodonaphthyl azide

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• 作者：Paridhi Gupta (1)
  Anuj Sharma (1)
  Viard Mathias (2)
  Yossef Raviv (2)
  Robert Blumenthal (3)
  Radha K Maheshwari (1)
  
  1. Department of Pathology ; Uniformed Services University of the Health Sciences ; Bethesda ; MD ; USA
  2. Basic Science Program ; Leidos Biomedical Research ; Inc. ; NCI Center for Cancer Research ; Frederick National Laboratory for Cancer Research ; Frederick ; MD ; USA
  3. Chemical Biology Lab ; Center for Cancer Research ; National Cancer Institute ; Frederick ; MD ; USA
  
• 关键词：Inactivated ; EMCV ; Non ; enveloped ; Iodonaphthyl azide
• 刊名：BMC Research Notes
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：8
• 期：1
• 全文大小：692 KB
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• 刊物主题：Biomedicine general; Medicine/Public Health, general; Life Sciences, general;
• 出版者：BioMed Central
• ISSN：1756-0500

文摘

Background A photoactive hydrophobic agent 1,5-iodonaphthyl-azide (INA), has been previously shown to completely inactivate the enveloped viruses. INA sequesters into the lipid bilayer of the virus envelope and upon UV-irradiation bind to the hydrophobic domains of the envelope glycoproteins. In our earlier study, we have shown that the Venezuelan equine encephalitis virus (VEEV) genomic RNA was also inactivated during the inactivation of the virus with INA. Findings In the present study, we evaluated if the RNA inactivation property of INA can be used to inactivate non-enveloped RNA viruses. Encephalomyocarditis virus (EMCV) was used as a model non-enveloped virus. Treatment with INA followed by UV-irradiation resulted in complete inactivation of EMCV. RNA isolated from INA-inactivated EMCV was non-infectious and INA was found to be associated with the viral RNA genome. INA-inactivated EMCV induced robust total antibody response. However binding capacity of INA-inactivated EMCV to neutralizing antibody was inhibited. Conclusion This is the first study to show that INA can completely inactivate non-enveloped virus. Our results suggest that the amino acid composition of the neutralizing epitope may interfere with the protective antibody response generated by the INA-inactivated non-enveloped virus.