Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies

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• 作者：Harshita Sharma (1)
  Yasunori Kinoshita (1)
  Seiichi Fujiu (1) (2)
  Shota Nomura (1)
  Mizuho Sawada (2)
  Shamim Ahmed (1) (6)
  Masaki Shibuya (3)
  Kosaku Shirai (3)
  Syota Takamatsu (4)
  Tsuyoshi Watanabe (4)
  Hitoshi Yamazaki (5)
  Ryohei Kamiyama (1)
  Tetsuya Kobayashi (1)
  Hidenao Arai (1)
  Miho Suzuki (1)
  Naoto Nemoto (1)
  Ki Ando (1) (7)
  Hidekazu Uchida (1)
  Koichiro Kitamura (2)
  Osamu Takei (3)
  Koichi Nishigaki (1)
  
  1. Department of Functional Materials Science ; Graduate School of Science and Engineering ; Saitama University ; 255 Shimo-okubo ; Saitama ; 338-8570 ; Japan
  2. Janusys Corporation ; Saitama Industrial Technology Center ; 3-12-18 Kamiaoki ; Kawaguchi ; Saitama ; 334-0844 ; Japan
  6. Department of Biochemistry and Molecular Biology ; Shahjalal University of Science and Technology ; Sylhet ; Bangladesh
  3. Lifetech Co. ; Ltd ; 4074 Miyadera ; Iruma City ; Saitama ; 358-0014 ; Japan
  4. Enplas Corporation ; 2-30-1 Namiki ; Kawaguchi City ; Saitama ; 332-0034 ; Japan
  5. Finetech Corporation ; 1-7-1 ; Asagaya-minami Suginami-ku ; Tokyo ; Japan
  7. Department of Electrical and Electronic Engineering ; Tokyo Denki University ; 5 Senjyu-Asahi-cho ; Adachi-ku ; Tokyo ; 120-8551 ; Japan
  
• 关键词：Microarray ; Multi ; parallel reactions ; Multi ; conditioner ; Lysozyme crystallization ; Microbiome analysis
• 刊名：BMC Biotechnology
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：14
• 期：1
• 全文大小：1,696 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Life Sciences
  Plant Breeding/Biotechnology
  Stem Cells
  Transgenics
  
• 出版者：BioMed Central
• ISSN：1472-6750

文摘

Background With the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip. Results In the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications. Conclusions The reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.