Fibronectin immobilization on to robotic-dispensed nanobioactive glass/polycaprolactone scaffolds for bone tissue engineering

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• 作者：Jong-Eun Won (1) (2)
  Miguel A. Mateos-Timoneda (3) (4)
  Oscar Castano (4)
  Josep A. Planell (4)
  Seog-Jin Seo (1) (2)
  Eun-Jung Lee (1) (2)
  Cheol-Min Han (1) (5)
  Hae-Won Kim (1) (2) (5)
  
  1. Institute of Tissue Regeneration Engineering (ITREN) ; Dankook University ; Cheonan ; 330-714 ; South Korea
  2. Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for Regenerative Medicine ; Dankook University ; Cheonan ; 330-714 ; South Korea
  3. Biomaterials for Regenerative Therapies ; Institute for Bioengineering of Catalonia (IBEC) ; Barcelona ; Spain
  4. CIBER de Bioingenier铆a ; Biomateriales y Nanomedicina (CIBER-BBN) ; Barcelona ; Spain
  5. Department of Biomaterials Science ; College of Dentistry ; Dankook University ; Cheonan ; 330-714 ; South Korea
  
• 关键词：Bone scaffolds ; Cell response ; Fibronectin ; Nanobioactive glass ; Nanocomposites ; Polycaprolactone
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：37
• 期：4
• 页码：935-942
• 全文大小：2,252 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Bioactive nanocomposite scaffolds with cell-adhesive surface have excellent bone regeneration capacities. Fibronectin (FN)-immobilized nanobioactive glass (nBG)/polycaprolactone (PCL) (FN-nBG/PCL) scaffolds with an open pore architecture were generated by a robotic-dispensing technique. The surface immobilization level of FN was significantly higher on the nBG/PCL scaffolds than on the PCL scaffolds, mainly due to the incorporated nBG that provided hydrophilic chemical-linking sites. FN-nBG/PCL scaffolds significantly improved cell responses, including initial anchorage and subsequent cell proliferation. Although further in-depth studies on cell differentiation and the in vivo animal responses are required, bioactive nanocomposite scaffolds with cell-favoring surface are considered to provide promising three-dimensional substrate for bone regeneration.