Metabolic engineering of Escherichia coli W3110 for the production of l-methionine
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- 作者：Hua Li ; Bao Shi Wang ; You Ran Li…
- 关键词：Escherichia coli ; l ; homoserine ; l ; methionine ; Metabolic engineering ; Biosynthesis obstacle
- 刊名：Journal of Industrial Microbiology & Biotechnology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：44
- 期：1
- 页码：75-88
- 全文大小：
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Microbiology; Biochemistry, general; Inorganic Chemistry; Genetic Engineering; Biotechnology; Bioinformatics;
- 出版者：Springer Berlin Heidelberg
- ISSN：1476-5535
- 卷排序：44
文摘
In this study, we constructed an l-methionine-producing recombinant strain from wild-type Escherichia coli W3110 by metabolic engineering. To enhance the carbon flux to methionine and derepression met regulon, thrBC, lysA, and metJ were deleted in turn. Methionine biosynthesis obstacles were overcome by overexpression of metAFbr (Fbr, Feedback resistance), metB, and malY under control of promoter pN25. Recombinant strain growth and methionine production were further improved by attenuation of metK gene expression through replacing native promoter by metK84p. Blocking the threonine pathway by deletion of thrBC or thrC was compared. Deletion of thrC showed faster growth rate and higher methionine production. Finally, metE, metF, and metH were overexpressed to enhance methylation efficiency. Compared with the original strain E. coli W3110, the finally obtained Me05 (pETMAFbr-B-Y/pKKmetH) improved methionine production from 0 to 0.65 and 5.62 g/L in a flask and a 15-L fermenter, respectively.