Hairy and Enhancer of Split 6 (Hes6) Deficiency in Mouse Impairs Neuroblast Differentiation in Dentate Gyrus Without Affecting Cell Proliferation and Integration into Mature Neurons

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• 作者：Sung Min Nam ; Yo Na Kim ; Jong Whi Kim…
• 关键词：Hes6 ; Neuronal differentiation ; Hippocampus ; Adult neurogenesis
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：36
• 期：1
• 页码：57-67
• 全文大小：3,111 KB
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• 作者单位：Sung Min Nam (1) (2) (3)
  Yo Na Kim (1) (2) (3)
  Jong Whi Kim (1) (2) (3)
  Dong Soo Kyeong (1) (2) (3)
  Seo Hyun Lee (1) (2) (3)
  Yeri Son (1) (2) (3)
  Jae Hoon Shin (1) (2) (3)
  Jaesang Kim (5)
  Sun Shin Yi (6)
  Yeo Sung Yoon (1) (2) (3)
  Je Kyung Seong (1) (2) (3) (4)
  
  1. Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 151-742, South Korea
  2. BK21 PLUS Program for Creative Veterinary Science Research and Research Institute for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea
  3. KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, 151-742, South Korea
  5. Department of Life Sciences and Ewha Research Center for Systems Biology, Ewha Womans University, Seoul, 120-750, South Korea
  6. Department of Biomedical Laboratory Science, College of Biomedical Sciences, Soonchunhyang University, Asan, 336-745, South Korea
  4. Interdisciplinary Program for Bioinformatics, Program for Cancer Biology and BIO-MAX Institute, Seoul National University, Seoul, 151-742, South Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Neurosciences
  Animal Anatomy, Morphology and Histology
  
• 出版者：Springer Netherlands
• ISSN：1573-6830

文摘

Hes6 is a member of the hairy-enhancer of split homolog (Hes) family of transcription factors and interacts with other Hes family genes. During development, Hes genes are expressed in neural stem cells and progenitor cells. However, the role of Hes6 in adult hippocampal neurogenesis remains unclear. We therefore investigated the effects of Hes6 on adult hippocampal neurogenesis, by comparing Hes6 knockout and wild-type mice. To this end, we immunostained for markers of neural stem cells and progenitor cells (nestin), proliferating cells (Ki67), post-mitotic neuroblasts and immature neurons (doublecortin, DCX), mature neuronal cells (NeuN), and astrocyte (S100β). We also injected 5-bromo-2′-deoxyuridine (BrdU) to trace the fate of mitotic cells. Nestin- and Ki67-positive proliferating cells did now show any significant differences between wild and knockout groups. Hes6 knockout negatively affects neuroblast differentiation based on DCX immunohistochemistry. On the contrary, the ratio of the BrdU and NeuN double-positive cells did not show any significance, even though it was slightly higher in the knockout group. These results suggest that Hes6 is involved in the regulation of neuroblast differentiation during adult neurogenesis, but does not influence integration into mature neurons. Keywords Hes6 Neuronal differentiation Hippocampus Adult neurogenesis