Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats
详细信息 查看全文
- 作者：Ming Fang ; Yun Yuan ; Parakalan Rangarajan ; Jia Lu ; Yajun Wu…
- 关键词：Scutellarin ; Cerebral ischemia ; Astrogliosis ; GFAP ; Notch ; Nestin ; Proinflammatory mediators ; TNC1
- 刊名：BMC Neuroscience
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：16
- 期：1
- 全文大小：4,860 KB
- 刊物主题：Neurosciences; Neurobiology; Animal Models;
- 出版者：BioMed Central
- ISSN：1471-2202
文摘
Background Scutellarin, an anti-inflammatory agent, effectively suppressed microglia activation in rats with middle cerebral artery occlusion (MCAO). Robust microglia activation, acute in onset, was followed by astrogliosis. This study was aimed to determine if scutellarin would also affect the reactive astrocytes that play an important role in tissue repair. Expression of GFAP and Notch-1 and its members: Notch receptor intracellular domain (NICD), and transcription factor hairy and enhancer of split-1 (HES-1), together with nestin and proinflammatory mediators was assessed by immunofluorescence staining in TNC1 astrocytes treated, respectively, with BV-2 conditioned medium (CM) and CM + lipopolysaccharide (LPS) (CM + L) serving as the controls, and conditioned medium derived from LPS-activated BV-2 cells pretreated with scutellarin (CM + SL). Study of the above biomarkers was then extended to reactive astrocytes in scutellarin injected MCAO rats.