Enrofloxacin and Probiotic Lactobacilli Influence PepT1 and LEAP-2 mRNA Expression in Poultry

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• 作者：Ivelina Pavlova ; Aneliya Milanova ; Svetla Danova…
• 关键词：Chicken ; Probiotics ; Enrofloxacin ; PepT1 mRNA ; LEAP ; 2 mRNA
• 刊名：Probiotics and Antimicrobial Proteins
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：8
• 期：4
• 页码：215-220
• 全文大小：
• 刊物主题：Chemistry/Food Science, general; Applied Microbiology; Microbiology; Protein Science; Nutrition;
• 出版者：Springer US
• ISSN：1867-1314
• 卷排序：8

文摘

Expression of peptide transporter 1 (PepT1) and liver-expressed antimicrobial peptide 2 (LEAP-2) in chickens can be influenced by food deprivation, pathological conditions and drug administration. Effect of three putative probiotic Lactobacillus strains and enrofloxacin on the expression of PepT1 and LEAP-2 mRNA was investigated in Ross 308 chickens. One-day-old chicks (n = 24) were allocated to following groups: control (without treatment); group treated with probiotics via feed; group treated with a combination of probiotics and enrofloxacin; and a group given enrofloxacin only. The drug was administered at a dose of 10 mg kg−1, via drinking water for 5 days. Samples from liver, duodenum and jejunum were collected 126 h after the start of the treatment. Expression levels of PepT1 and LEAP-2 were determined by real-time polymerase chain reaction and were statistically evaluated by Mann–Whitney test. Enrofloxacin administered alone or in combination with probiotics provoked a statistically significant up-regulation of PepT1 mRNA levels in the measured organ sites. These changes can be attributed to a tendency of improvement in utilization of dietary peptide and in body weight gain. LEAP-2 mRNA expression levels did not change significantly in enrofloxacin-treated chickens in comparison with control group.