The detection and expression of enterotoxinencoding lth gene among Klebsiella spp. isolated from diarrhoea

详细信息    查看全文

• 作者：Adriana Janczura (1)
  Danuta Smutnicka (1)
  Adam Junka (1)
  Gra?yna Go?ciniak (1)
  
• 关键词：Klebsiella strains ; Intestinal tract ; Enterotoxin production ; Diarrhoea
• 刊名：Central European Journal of Biology
• 出版年：2013
• 出版时间：February 2013
• 年：2013
• 卷：8
• 期：2
• 页码：121-129
• 全文大小：780KB
• 参考文献：1. Klipstein F.A., Engert R.F., Enterotoxigenic intestinal bacteria in tropical sprue. III. Preeliminary characterization of Klebsiella pneumoniae enterotoxin, J. Infect. Dis., 1975, 132, 200-03 CrossRef
  2. Klipstein F.A., Horowitz I.R., Engert R.F., Schenk E.A., Effect of Klebsiella pneumoniae enterotoxin on intestinal transport in the rat, J. Clin. Invest., 1975, 56, 799-07 CrossRef
  3. Klipstein F.A., Engert R., Purification and properties of Klebsiella pneumoniae heat-stable enterotoxin, Infect. Immun., 1976, 13, 373-81
  4. Klipstein F.A., Engert R.F., Houghten R.A., Immunological properties of purified Klebsiella pneumoniae heat-stabile enterotoxin, Infect. Immun., 1983, 42, 838-41
  5. Koo F.C.W., Stein M.D., Detection of an extracellular toxin produced by burn isolates of Klebsiella pneumonia, Current Microbiol., 1986, 13, 23-7 CrossRef
  6. Speirs J.I., Stavric S., Konowalchuk J., Assay of Escherichia coli heat-labile enterotoxin with Vero cells, Infect. Immun., 1977, 16, 617-22
  7. Berberov E.M., Zhou Y., Francis D.H., Scott M.A., Kachman S.D., Moxley R.A., Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic Escherichia coli that produces multiple enterotoxins, Infect. Immun., 2004, 72, 3914-924 CrossRef
  8. Alessio M., Albano F., Tarallo L., Guarino A., Interspecific plasmid transfer and modification of heat-stable enterotoxin expression by Klebsiella pneumoniae from infants with diarrhea, Pediatr. Res., 1993, 33, 205-08 CrossRef
  9. Guarino A., Capano G., Malamisura B., Alessio M., Guandalini S., Rubino A., Production of Escherichia coli STa-like heat-stable enterotoxin by Citrobacter freundii isolated from humans, J. Clin. Microbiol., 1987, 25, 110-14
  10. Nair G.B., Takeda Y., The heat-stable enterotoxins, Microb. Pathog., 1998, 24, 123-31 CrossRef
  11. Pobucewicz A., Czernomysy-Furowicz D., Characteristics of Citrobacter freundii strain with special focus on its ability to produce toxins [Charakterystyka Citrobacter freundii, ze szczególnym uwzgl?dnieniem ich zdolno?ci do wytwarzania toksyn], Post. Mikrobiol., 2010, 40, 43-6, (in Polish)
  12. Neill R.J., Twiddy E.M., Holmes R.K., Synthesis of plasmid-coded heat-labile enterotoxin in wild-type and hypertoxinogenic strains of Escherichia coli and in other genera of Enterobacteriaceae, Infect. Immun., 1983, 41, 1056-061
  13. Panigraphi D., Roy P., Chakrabarti A., Enterotoxigenic Klebsiella pneumoniae in acute childhood diarrhea, Indian J. Med. Res., 1991, 93, 293-96
  14. Mavzioutov A., Bondarenko V., Baymiev A.N., Baymiev A., Escherichia enterotoxins genes are detectable among clinical isolates of Klebsiella ssp. and Proteus spp., Clin. Microbiol. Infect., 2002, 8,Suppl.1, Abstract P614
  15. Burgess M.N., Cowley C.M., Melling J., Mullan N.A., Newsome P.M., Assay of the heat-labile enterotoxin of Escherichia coli in infant rabbits, J. Med. Microbiol., 1979, 12, 291-02 CrossRef
  16. Donta S.T., Moon H.W., Whipp S.C., Detection of heat-labile Escherichia coli enterotoxin with the use of adrenal cells in tissue culture, Science, 1974, 183, 334-36 CrossRef
  17. Guerrant R.L., Brunton L.L., Schnaitman T.C., Rebhun L.L., Gilman A.G., Cyclic adenosine monophosphate and alteration of Chinese Hamster Ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli, Infect. Immun., 1974, 10, 320-27
  18. Nataro J.P., Kaper J.B., Diarrheagenic Escherichia coli, Clin. Microb. Rev., 1998, 11, 142-01
  19. Hedge A., Bhat G.K., Mallya S., Effect of stress on production of heat-labile enterotoxin by Escherichia coli, Indian J. Med. Microbiol., 2009, 27, 325-28 CrossRef
  
• 作者单位：Adriana Janczura (1)
  Danuta Smutnicka (1)
  Adam Junka (1)
  Gra?yna Go?ciniak (1)
  
  1. Department of Microbiology, Medical University of Wroclaw, 50-368, Wroclaw, Poland
  
• ISSN：1644-3632

文摘

Background Currently, when isolated from diarrheal stool, Klebsiella spp., a member of the Enterobacteriaceae family, is treated as a physiological flora even when it occurs in monoculture. The possibility of transfer of virulence plasmids from diarrheal E.coli strains to the Klebsiella strains inhabiting the gastro-intestinal tract provokes a question of whether these bacteria can be the etiological factor of diarrhoea. Methodology 152 Klebsiella spp. strains were used in this study. The presence of the lth gene coding heat-labile enterotoxin (LT) in the plasmids of Klebsiella spp. strains was detected using PCR, whereas the expression of the toxin was evaluated by measuring the cytopathic effect induced by the LT toxin in Chinese Hamster Ovary (CHO) cell lines. Results The lth gene was detected in 8.5% of the investigated Klebsiella strains. 77% of these strains were able to produce the cytopathic effect in the CHO cell lines. Surprisingly, part of the strains with no lth gene detected, were also able to cause above mentioned effect. Conclusions The results of our studies suggest that Klebsiella spp. isolated from the gastro-intestinal tract may be an etiologic factor of diarrhoeas.