Engineering geminivirus resistance in Jatropha curcus

详细信息    查看全文

• 作者：Jian Ye (1) (2)
  Jing Qu (1)
  Hui-Zhu Mao (1)
  Zhi-Gang Ma (1)
  Nur Estya Binte Rahman (1)
  Chao Bai (2)
  Wen Chen (1)
  Shu-Ye Jiang (1)
  Srinivasan Ramachandran (1)
  Nam-Hai Chua (3)
  
  1. Temasek Life Sciences Laboratory ; NO.1 Research Link ; National University of Singapore ; Singapore ; 117604 ; Singapore
  2. State Key Laboratory of Plant Genomics ; Institute of Microbiology ; Chinese Academy of Sciences ; NO.1 Beichen West Road ; Beijing ; 100101 ; China
  3. Laboratory of Plant Molecular Biology ; Rockefeller University ; 1230 York Avenue ; New York ; NY ; 10021 ; USA
  
• 关键词：Biodiesel ; Indian cassava mosaic virus ; Jatropha curcus ; Virus resistance ; Transgenic
• 刊名：Biotechnology for Biofuels
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：2,095 KB
• 参考文献：1. Raj, SK, Snehi, SK, Kumar, S, Khan, MS, Pathre, U (2008) First molecular identification of a begomovirus in India that is closely related to Cassava mosaic virus and causes mosaic and stunting of Jatropha curcas L. Australasian Plant Dis Notes 3: pp. 69-72 CrossRef
  2. Aswatha Narayana, DS, Rangawarmy, KT, Shankarappa, KS, Maruthi, MN, Reddy, CNL, Rekha, AR, Murthy, KVK (2007) Distinct begomoviruses closely related to Cassava Mosaic Viruses cause Indian Jatropha Mosaic Disease. Int J Virol 3: pp. 1-11 CrossRef
  3. Gao, S, Qu, J, Chua, NH, Ye, J (2010) A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. Arch Virol 155: pp. 607-612 CrossRef
  4. Shehi, S, Srivastava, A, Raj, S (2012) Biological characterization and complete genome sequence of a possible strain of Indian cassava mosaic virus from Jatropha curcas in India. J Phytopathol 160: pp. 547-553 CrossRef
  5. Ramkat, RC, Calari, A, Maghuly, F, Laimer, M (2011) Biotechnological approaches to determine the impact of viruses in the energy crop plant Jatropha curcas. Virol J 8: pp. 386 CrossRef
  6. Kashina, BD, Alegbejo, MD, Banwo, OO, Nielsen, SL, Nicolaisen, M (2013) Molecular identification of a new begomovirus associated with mosaic disease of Jatropha curcas L. in Nigeria. Arch Virol 158: pp. 511-514 CrossRef
  7. Wang, G, Sun, Y, Xu, R, Qu, J, Tee, C, Jiang, X, Ye, J (2014) DNA-A of a highly pathogenic Indian cassava mosaic virus isolated from Jatropha curcas causes symptoms in Nicotiana benthamiana. Virus Genes 48: pp. 402-405 CrossRef
  8. Brown, J, Fauquet, C, Briddon, R, Zerbini, M, Moriones, E, Navas-Castillo, J Geminiviridae. In: King, A, Adams, M, Carstens, E, Lefkowitz, E eds. (2012) Virus Taxonomy - Ninth Report of the International Committee on Taxonomy of Viruses. Associated Press, Elsevier Inc, London, Waltham, San Diego, pp. 351-373
  9. Ahuja, SL, Monga, D, Dhayal, LS (2007) Genetics of resistance to cotton leaf curl disease in Gossypium hirsutum L. under field conditions. J Hered 98: pp. 79-83 CrossRef
  10. Li, MR, Li, HQ, Jiang, HW, Pan, XP, Wu, GJ (2008) Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas. Plant Cell Tiss Organ Cult 92: pp. 173-181 CrossRef
  11. Pan, J, Fu, Q, Xu, Z (2010) Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection. Afr J Biotechnol 9: pp. 6477-6481
  12. Kajikawa, M, Morikawa, K, Inoue, M, Widyastuti, U, Suharsono, S, Yokota, A, Akashi, K (2012) Establishment of bispyribac selection protocols for Agrobacterium tumefaciens-and Agrobacterium rhizogenes-mediated transformation of the oil seed plant Jatropha curcas L. (Special Issue: Jatropha research: a new frontier for biofuel development). Plant Biotechnol J 29: pp. 145-153 CrossRef
  13. Qu, J, Mao, HZ, Chen, W, Gao, SQ, Bai, YN, Sun, YW, Geng, YF, Ye, J (2012) Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. Biotechnol Biofuels 5: pp. 10 CrossRef
  14. Vanderschuren, H, Alder, A, Zhang, P, Gruissem, W (2009) Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava. Plant Mol Biol 70: pp. 265-272 CrossRef
  15. Bonfim, K, Faria, JC, Nogueira, EO, Mendes, EA, Aragao, FJ (2007) RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered Ahuja (Phaseolus vulgaris). Mol Plant Microbe Interact 20: pp. 717-726 CrossRef
  16. Abhary, MK, Anfoka, GH, Nakhla, MK, Maxwell, DP (2006) Post-transcriptional gene silencing in controlling viruses of the Tomato yellow leaf curl virus complex. Arch Virol 151: pp. 2349-2363 CrossRef
  17. Zhang, P, Gruissem, W (2003) Efficient replication of cloned African cassava mosaic virus in cassava leaf disks. Virus Res 92: pp. 47-54 CrossRef
  18. Vanderschuren, H, Stupak, M, Futterer, J, Gruissem, W, Zhang, P (2007) Engineering resistance to geminiviruses鈥搑eview and perspectives. Plant Biotechnol J 5: pp. 207-220 7652.2006.00217.x" target="_blank" title="It opens in new window">CrossRef
  19. Zuo, JR, Niu, QW, M酶ller, SG, Chua, NH (2001) Chemical-regulated, site-specific DNA excision in transgenic plants. Nature Biotech 19: pp. 157-161 CrossRef
  20. Guo, HS, Fei, JF, Xie, Q, Chua, NH (2003) A chemical-regulated inducible RNAi system in plants. Plant J 34: pp. 383-392 CrossRef
  21. Tamura, K, Dudley, J, Nei, M, Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24: pp. 1596-1599 CrossRef
  22. Chen, W, Qian, Y, Wu, X, Sun, Y, Wu, X, Cheng, X (2014) Inhibiting replication of begomoviruses using artificial zinc finger nucleases that target viral-conserved nucleotide motif. Virus Genes 48: pp. 494-501 CrossRef
  23. Shepherd, DN, Mangwende, T, Martin, DP, Bezuidenhout, M, Kloppers, FJ, Carolissen, CH, Monjane, AL, Rybicki, EP, Thomson, JA (2007) Maize streak virus-resistant transgenic maize: a first for Africa. Plant Biotechnol J 5: pp. 759-767 7652.2007.00279.x" target="_blank" title="It opens in new window">CrossRef
  24. Reyes, MI, Nash, TE, Dallas, MM, Ascencio-Ibanez, JT, Hanley-Bowdoin, L (2013) Peptide aptamers that bind to geminivirus replication proteins confer a resistance phenotype to tomato yellow leaf curl virus and tomato mottle virus infection in tomato. J Virol 87: pp. 9691-9706 CrossRef
  25. Safarnejad, MR, Fischer, R, Commandeur, U (2009) Recombinant-antibody-mediated resistance against Tomato yellow leaf curl virus in Nicotiana benthamiana. Arch Virol 154: pp. 457-467 CrossRef
  26. Bonas, U, Lahaye, T (2002) Plant disease resistance triggered by pathogen-derived molecules: refined models of specific recognition. Curr Opin Microbiol 5: pp. 44-50 CrossRef
  27. Prins, M, Laimer, M, Noris, E, Schubert, J, Wassenegger, M, Tepfer, M (2008) Strategies for antiviral resistance in transgenic plants. Mol Plant Pathol 9: pp. 73-83
  28. Vu, TV, Choudhury, NR, Mukherjee, SK (2013) Transgenic tomato plants expressing artificial microRNAs for silencing the pre-coat and coat proteins of a begomovirus, Tomato leaf curl New Delhi virus, show tolerance to virus infection. Virus Res 172: pp. 35-45 CrossRef
  29. Qu, J, Ye, J, Fang, R (2007) Artificial microRNA-mediated virus resistance in plants. J Virol 81: pp. 6690-6699 CrossRef
  30. Mubin, M, Hussain, M, Briddon, RW, Mansoor, S (2011) Selection of target sequences as well as sequence identity determine the outcome of RNAi approach for resistance against cotton leaf curl geminivirus complex. Virol J 8: pp. 122 CrossRef
  31. Ramesh, SV, Mishra, AK, Praveen, S (2007) Hairpin RNA-mediated strategies for silencing of tomato leaf curl virus AC1 and AC4 genes for effective resistance in plants. Oligonucleotides 17: pp. 251-257 CrossRef
  32. Nahid, N, Amin, I, Briddon, RW, Mansoor, S (2011) RNA interference-based resistance against a legume mastrevirus. Virol J 8: pp. 499 CrossRef
  33. Aragao, FJ, Faria, JC (2009) First transgenic geminivirus-resistant plant in the field. Nature Biotech 27: pp. 1086-1088 CrossRef
  34. Yi, C, Zhang, S, Liu, X, Bui, HT, Hong, Y (2010) Does epigenetic polymorphism contribute to phenotypic variances in Jatropha curcas L.?. BMC Plant Biol 10: pp. 259 CrossRef
  35. Ye, J, Qu, J, Bui, HT, Chua, NH (2009) Rapid analysis of Jatropha curcas gene functions by virus-induced gene silencing. Plant Biotechnol J 7: pp. 964-976 7652.2009.00457.x" target="_blank" title="It opens in new window">CrossRef
  36. Prisco, GD, Zhang, X, Pennacchio, F, Caprio, E, Li, J, Evans, JD, Degrandi-Hoffman, G, Hamilton, M, Chen, YP (2011) Dynamics of persistent and acute deformed wing virus infections in honey bees, Apis mellifera. Viruses 3: pp. 2425-2441 CrossRef
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Plant Breeding/Biotechnology
  Renewable and Green Energy
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1754-6834

文摘

Background Jatropha curcus is a good candidate plant for biodiesel production in tropical and subtropical regions. However, J. curcus is susceptible to the geminivirus Indian cassava mosaic virus (ICMV), and frequent viral disease outbreaks severely limit productivity. Therefore the development of J. curcus to carry on durable virus resistance remains crucial and poses a major biotechnological challenge. Results We generated transgenic J. curcus plants expressing a hairpin, double-stranded (ds) RNA with sequences homologous to five key genes of ICMV-Dha strain DNA-A, which silences sequence-related viral genes thereby conferring ICMV resistance. Two rounds of virus inoculation were conducted via vacuum infiltration of ICMV-Dha. The durability and heritability of resistance conferred by the dsRNA was further tested to ascertain that T1 progeny transgenic plants were resistant to the ICMV-SG strain, which shared 94.5% nucleotides identity with the ICMV-Dha strain. Quantitative PCR analysis showed that resistant transgenic lines had no detectable virus. Conclusions In this study we developed transgenic J. curcus plants to include a resistance to prevailing geminiviruses in Asia. These virus-resistant transgenic J. curcus plants can be used in various Jatropha breeding programs.