Poly(Ethylene Glycol) Hydrogel Scaffolds Containing Cell-Adhesive and Protease-Sensitive Peptides Support Microvessel Formation by Endothelial Progenitor Cells

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• 作者：Erica B. Peters ; Nicolas Christoforou…
• 关键词：Vascularization ; Tissue engineering ; Endothelial progenitor cells ; Biomaterials ; Hydrogels
• 刊名：Cellular and Molecular Bioengineering
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：9
• 期：1
• 页码：38-54
• 全文大小：11,903 KB
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• 作者单位：Erica B. Peters (1)
  Nicolas Christoforou (2)
  Kam W. Leong (3)
  George A. Truskey (1)
  Jennifer L. West (1)
  
  1. Department of Biomedical Engineering, Duke University, Fitzpatrick CIEMAS Building, Room 1427, Box 90281, Durham, NC, 27708, USA
  2. Department of Biomedical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, United Arab Emirates
  3. Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Avenue, Mail Code 8904, New York, NY, 10027, USA
  
• 刊物类别：Engineering
• 刊物主题：Biomedical Engineering
  Mechanics
  Continuum Mechanics and Mechanics of Materials
  Biophysics and Biomedical Physics
  Cell Biology
  
• 出版者：Springer New York
• ISSN：1865-5033

文摘

The development of stable, functional microvessels remains an important obstacle to overcome for tissue engineered organs and treatment of ischemia. Endothelial progenitor cells (EPCs) are a promising cell source for vascular tissue engineering as they are readily obtainable and carry the potential to differentiate towards all endothelial phenotypes. The aim of this study was to investigate the ability of human umbilical cord blood-derived EPCs to form vessel-like structures within a tissue engineering scaffold material, a cell-adhesive and proteolytically degradable polyethylene glycol hydrogel. EPCs in co-culture with angiogenic mural cells were encapsulated in hydrogel scaffolds by mixing with polymeric precursors and using a mild photocrosslinking process to form hydrogels with homogeneously dispersed cells. EPCs formed 3D microvessels networks that were stable for at least 30 days in culture, without the need for supplemental angiogenic growth factors. These 3D EPC microvessels displayed aspects of physiological microvasculature with lumen formation, expression of endothelial cell proteins (connexin 32, VE-cadherin, eNOS), basement membrane formation with collagen IV and laminin, perivascular investment of PDGFR-β and α-SMA positive cells, and EPC quiescence (<1% proliferating cells) by 2 weeks of co-culture. Our findings demonstrate the development of a novel, reductionist system that is well-defined and reproducible for studying progenitor cell-driven microvessel formation. Keywords Vascularization Tissue engineering Endothelial progenitor cells Biomaterials Hydrogels