L-methionine reduces oxidant stress in endothelial cells: Role of heme oxygenase-1, ferritin, and nitric oxide

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• 作者：Kati Erdmann (1)
  Nina Grosser (1)
  Henning Schr?der (1)
  
• 关键词：L ; methionine ; heme oxygenase ; 1 ; ferritin ; micrountrients ; antioxidant
• 刊名：The AAPS Journal
• 出版年：2005
• 出版时间：March 2005
• 年：2005
• 卷：7
• 期：1
• 页码：E195-E200
• 全文大小：235KB
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• 作者单位：Kati Erdmann (1)
  Nina Grosser (1)
  Henning Schr?der (1)
  
  1. Department of Pharmacology and Toxicology, School of Pharmacy, Martin Luther University, 06099, Halle (Salle), Germany
  
• ISSN：1550-7416

文摘

The amino acid L-methionine is known to exert antioxidant effects by as yet unidentified mechanisms. In the present study, L-methionine led to a concentration-dependent induction of the antioxidant protein heme oxygenase-1 (HO-1) and ferritin in cultured endothelial cells (ECV 304). HO-1 protein expression was accompanied by an increased catalytic activity of the enzyme. Long-term pre-incubation of endothelial cells with L-methionine reduced NADPH-mediated radical formation by up to 60%. The antioxidant effect of L-methionine was mimicked by the HO-1 product bilirubin, which suppressed free radical formation and almost completely. Reduction of superoxide generation by L-methionine was inhibited in the presence of the nitric oxide (NO) synthase inhibitor L-NMMA, suggesting the involvement of endogenous NO in L-methionine-dependent cytoprotection. These findings demonstrate that L-methionine reduces free radical formation in endothelial cells, possibly through induction of heme oxygenase-1 and ferritin. This novel, indirect antioxidant action might be relevant for the preventive potential of methionine and methionine rich diets under conditions of inflammation and oxidative stress.