Long-Term Cultivation of Primmorphs from Freshwater Baikal Sponges Lubomirskia baikalensis

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• 作者：Lubov I. Chernogor (1) lchernogor@mail.ru
  Natalia N. Denikina (1)
  Sergey I. Belikov (1)
  Alexander V. Ereskovsky (23)
• 关键词：Freshwater sponges – Lake Baikal – Lubomirskia baikalensis – Dissociated cells – Primmorphs – Long ; term cultivation
• 刊名：Marine Biotechnology
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：13
• 期：4
• 页码：782-792
• 全文大小：828.3 KB
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• 作者单位：1. Laboratory of Analytical Bioorganic Chemistry, Limnological Institute of the Siberian Branch of Russian Academy of Sciences, 3 Ulan-Batorskaya St., Irkutsk, 664033 Russia2. Department of Embryology, Faculty of Biology and Soils, Saint-Petersburg State University, Universitetskaja nab. 7/9, St. Petersburg, 199034 Russia3. Centre d’Océanologie de Marseille, Station marine d’Endoume - CNRS UMR 6540-DIMAR, rue de la Batterie des Lions, Marseille, 13007 France
• 刊物类别：Earth and Environmental Science
• 刊物主题：Earth sciences
  Oceanography
  
• 出版者：Springer New York
• ISSN：1436-2236

文摘

The work was aimed at performing long-term cultivation of primmorphs in vitro from freshwater sponge Lubomirskia baikalensis (Pallas 1776), collected from Lake Baikal, obtaining its long-term primmorph culture in both natural (NBW) and artificial (ABW) Baikal water and at identifying the impact of different environmental factors on formation and growth of primmorphs. The first fine aggregates of L. baikalensis are formed in vitro 10–15 min after dissociation of sponge cells. Epithelization of aggregates begins 4 h later after the dissociation. Young primmorphs are formed 1 or 2 days later. The surface of primmorphs is covered with a layer of exopinacocytes. The primmorphs remain viable for more than 10 months at 3–6°C. Over 50% of primmorphs in NBW and 25% in ABW are attached to the substrate and grow like adult sponges. Thus, the long-term primmorph cultivation in vitro allows the creation of a controlled live model system under experimental conditions. The results of this work will allow the creation of a cell culture collection of Baikal freshwater sponges for studying morphogenesis of primmorphs during cultivation at different stages and transdifferentiation of their cells, physiological functions of sponge cells, processes of spiculogenesis, identification of proteins involved in biomineralization process, decoding of their genes, as well as a spectrum of secondary metabolites.