Identification and Characterization of a T-DNA Promoter Trap Line of Arabidopsis thaliana Uncovers an Embryo Sac-Specific Bi-directional Promoter

详细信息    查看全文

• 作者：Isha Sharma ; Ramamurthy Srinivasan…
• 关键词：Arabidopsis ; Embryo sac ; Endosperm ; Female gametophyte ; Gametogenesis ; Pectin methylesterase inhibitor
• 刊名：Plant Molecular Biology Reporter
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：33
• 期：5
• 页码：1404-1412
• 全文大小：2,972 KB
• 参考文献：Banerjee J, Sahoo DK, Dey N, Houtz RL, Maiti IB (2013) An intergenic region shared by At4g35985 and At4g35987 in Arabidopsis thaliana is a tissue specific and stress inducible bidirectional promoter analyzed in transgenic Arabidopsis and tobacco plants. PLoS ONE 8(11):e79622. doi:10.-371/?journal.?pone.-079622 PubMed Central CrossRef PubMed
  Bhalothia P, Alok A, Mehrotra S (2013) AACA element negatively regulates expression of protein phosphatase 2c (pp2c) like promoter in Arabidopsis thaliana. Am J Plant Sci 4:549-54CrossRef
  Bondino HG, Valle EM (2009) A small intergenic region drives exclusive tissue-specific expression of the adjacent genes in Arabidopsis thaliana. BMC Mol Biol 10:1471-199CrossRef
  Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735-43CrossRef PubMed
  Coutu C, Brandle J, Brown D, Brown K, Miki B, Simmonds J, Hegedus DD (2007) pORE: a modular binary vector series suited for both monocot and dicot plant transformation. Transgenic Res 16:771-81CrossRef PubMed
  Foster R, Izawa T, Chua NH (1994) Plant bZIP proteins gather at ACGT elements. FASEB J 8:192-00PubMed
  Gowik U, Burscheidt J, Akyildiz M, Schlue U, Koczor M, Streubel M, Westhoff P (2004) cis-Regulatory elements for mesophyll-specific gene expression in the C4 plant Flaveria trinervia, the promoter of the C4 phosphoenolpyruvate carboxylase gene. Plant Cell 16:1077-090PubMed Central CrossRef PubMed
  Higo K, Ugawa Y, Iwamoto M, Korenaga T (1999) Plant cis-acting regulatory DNA elements (PLACE) database. Nucleic Acids Res 27:297-00PubMed Central CrossRef PubMed
  Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 13:3901-907
  Kubo T, Fujita M, Takahashi H, Nakazono M, Tsutsumi N, Kurata N (2013) Transcriptome analysis of developing ovules in rice isolated by laser microdissection. Plant Cell Physiol 54:750-65CrossRef PubMed
  Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Peer YV, Rouze P, Rombauts S (2002) Plant CARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res 30:325-27PubMed Central CrossRef PubMed
  Li ZT, Jayasankar S, Gray DJ (2004) Bi-directional duplex promoters with duplicated enhancers significantly increase transgene expression in grape and tobacco. Transgenic Res 13:143-54CrossRef PubMed
  Lindsey K, Wei W, Clarke MC, McArdle HF, Rooke LM, Topping JF (1993) Tagging genomic sequences that direct transgene expression by activation of a promoter trap in plants. Transgenic Res 2:33-7CrossRef PubMed
  Mitra A, Han J, Zhang ZJ, Mitra A (2009) The intergenic region of Arabidopsis thaliana cab1 and cab2 divergent genes functions as a bidirectional promoter. Planta 229:1015-022CrossRef PubMed
  Mollier P, Hoffmann B, Orsel M, Pelletier G (2000) Tagging of a cryptic promoter that confers root-specific GUS expression in Arabidopsis thaliana. Plant Cell Rep 19:1076-083CrossRef
  Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-97CrossRef
  Okresz L, Mathe C, Horvath E, Schell J, Koncz C, Szabados L (1998) T-DNA trapping of a cryptic promoter identifies an ortholog of highly conserved SNZ growth arrest response genes in Arabidopsis. Plant Sci 138:217-28CrossRef
  Plesch G, Kamann E, Mueller RB (2000) Cloning regulatory sequences mediating guard cell-specific gene expression. Gene 249:83-9CrossRef PubMed
  Pratibha P, Singh SK, Sharma I, Kumar R, Srinivasan R, Bhat SR, Ahuja PS, Sreenivasulu Y (2013) Characterization of a T-DNA promoter trap line of Arabidopsis thaliana uncovers a cryptic bi-directional promoter. Gene 524:22-7CrossRef PubMed
  Shin R, Kim MJ, Paek KH (2003) The CaTin1 (Capsicum annuum TMV-induced clone 1) and CaTin1- genes are linked head-to-head and share a bidirectional promoter. Plant Cell Physiol 44:549-54CrossRef PubMed
  Sivanandan C, Sujatha TP, Prasad AM, Resminath R, Thakare D, Bhat SR, Srinivasan R (2005) T-DNA tagging and characterization of a cryptic root-specific promoter in Arabidopsis. Biochem Biophys Acta 1731:202-08PubMed
  Stalberg K, Ellerst?m M, Ezcurra I, Ablov S, Rask L (1996) Disruption of an overlapping E-box/ABRE motif abolished high transcription of the napA storage-protein promoter in transgenic Brassica napus seeds. Planta 199:515-19CrossRef PubMed
  Tang W, Perry SE (2003) Binding site selection for the plant MADS domain protein AGL15: an in vitro and in vivo study. J Biol Chem 278:28154-8159CrossRef PubMed
  Trinklein ND, Aldred SF, Hartman SJ, Schroeder DI, Otillar RP, Myers RM (2004) An abundance of bidirectional promoters in the human genome. Genome Res 14:62-6PubMed Central CrossRef PubMed
  Vedel V, Scotti I (2011) Promoting the promoter. Plant Sci 180:182-8
• 作者单位：Isha Sharma (1)
  Ramamurthy Srinivasan (2)
  Paramvir Singh Ahuja (1)
  Shripad Ramachandra Bhat (2)
  Yelam Sreenivasulu (1)
  
  1. Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, 176061, Himachal Pradesh, India
  2. National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

The Arabidopsis thaliana promoter trap line GUS-650 showing uidA reporter gene expression specifically in mature embryo sacs was found to carry transfer DNA (T-DNA) insertion in the 5-untranslated region (UTR) of At3g17150. At3g17140 and At3g17150 are a pair of head-to-head-oriented genes separated by a 668-bp intergenic region encoding for a putative pectin methyl esterase inhibitor protein. In the GUS-650 line, uidA reporter gene was inserted in head-to-head orientation to the At3g17140 gene. Presence of At3g17140 transcripts in the GUS-650 line indicated that T-DNA insertion did not affect this gene and the 668-bp intergenic region functions as a bi-directional promoter. The 672-bp fragment separating the uidA and At3g17140 genes was cloned and placed in forward or reverse orientation next to the uidA gene. Transgenic Arabidopsis plants carrying either of these constructs showed β-glucuronidase (GUS) activity in the ovules confirming that the 672-bp sequence is a bi-directional promoter specific to ovules. These results were also supported by the presence of cis-acting motifs such as TATA box, CAAT box, seed storage protein element (CAACAC), endosperm-specific elements (AACA), Skn-1 motif (GTCAT) and bZIP transcription factor binding element (ACGT) within the 672-bp sequence in both orientations. The significance of a promoter trap approach and the utility of such a promoter are discussed. Keywords Arabidopsis Embryo sac Endosperm Female gametophyte Gametogenesis Pectin methylesterase inhibitor