Novel resistance mechanism of barley chlorina f104 antenna mutant against photoinhibition: possible role of new identified chloroplastic cpNrp protein

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• 作者：Mari谩n Bresti膷 ; Marek 沤iv膷谩k ; Miroslav 膸atko…
• 关键词：Chlorophyll fluorescence ; Mass spectrometry ; Photooxidative stress ; Proteomics ; Two ; dimensional electrophoresis
• 刊名：Theoretical and Experimental Plant Physiology
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：27
• 期：1
• 页码：75-85
• 全文大小：
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• 作者单位：Mari谩n Bresti膷 (1)
  Marek 沤iv膷谩k (1)
  Miroslav 膸atko (1)
  Katar铆na Ol拧ovsk谩 (1)
  Oksana Sytar (1) (2)
  Hongbo Shao (3) (4)
  
  1. Department of Plant Physiology, Slovak Agricultural University in Nitra, Tr. A. Hlinku 2, 949 76, Nitra, Slovak Republic
  2. Department of Plant Physiology and Ecology, Taras Shevchenko National University of Kyiv, Volodymyrs鈥檏a St. 64, Kiev, 01601, Ukraine
  3. Key Laboratory of Coastal Biology & Bioresources Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, 264003, China
  4. Institute for Life Sciences, Qingdao University of Science & Technology, Zhengzhou Road 53, Qingdao, 266042, China
  
• 刊物类别：Life Sciences, general;
• 刊物主题：Life Sciences, general;
• 出版者：Springer International Publishing
• ISSN：2197-0025

文摘

Protein with NmrA domain serving as the receptor for oxidized NAD+/NADP was identified in chloroplasts of spring barley mutant chlorina f104. NmrA was upregulated under environmental stresses in comparison to wild type plants. Following bioinformatic analysis revealed the nuclear localization signal in this protein. Pathways for the protein transport from cytoplasm into the chloroplast are described, but the mechanism of protein transport from chloroplast to nucleus, where the gene transcription can be possibly affected, is not clear. This protein named as cpNrp (chloroplastic NmrA related protein) is probably a member of new protein family and can serve as chloroplastic redox receptor. Consequently, cpNrp transported as a signal molecule into the nucleus and influences on the gene transcription under environmental stresses (high light radiation). It can be suggested that the presence of these proteins in chloroplasts of chlorina f104 barley mutant is related to up-regulation of photoprotective mechanisms, contributing to maintenance of high resistance against photooxidative damage in conditions of excessive light intensity. Keywords Chlorophyll fluorescence Mass spectrometry Photooxidative stress Proteomics Two-dimensional electrophoresis