HvNax3¡ªa locus controlling shoot sodium exclusion derived from wild barley (Hordeum vulgare ssp. spontaneum)

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• 作者：Yuri Shavrukov ; Narendra K. Gupta ; Junji Miyazaki ; Manahil N. Baho ; Kenneth J. Chalmers ; Mark Tester ; Peter Langridge and Nicholas C. Collins
• 关键词：Sodium transport ; Barley ; H. vulgare ssp. spontaneum ; Salinity tolerance ; Pyrophosphatase
• 刊名：Functional & Integrative Genomics
• 出版年：2010
• 出版时间：May, 2010
• 年：2010
• 卷：10
• 期：2
• 页码：277-291
• 全文大小：527.7 KB

文摘

Previous work identified the wild barley (Hordeum vulgare ssp. spontaneum) accession CPI-71284-48 as being capable of limiting sodium (Na+) accumulation in the shoots under saline hydroponic growth conditions. Quantitative trait locus (QTL) analysis using a cross between CPI-71284-48 and a selection of the cultivated barley (H. vulgare ssp. vulgare) cultivar Barque (Barque-73, a moderate Na+ excluder) attributed the control of the Na+ exclusion trait from CPI-71284-48 to a single locus on the short arm of chromosome 7H, which was named HvNax3. The locus reduced shoot Na+ accumulation by 10–25 % in plants grown in 150 mM NaCl. Markers generated using colinearity with rice and Brachypodium, together with the analysis of introgression lines and F₂ and F₃ families, enabled HvNax3 to be mapped to a 1.3-cM interval. Genes from the corresponding rice and Brachypodium intervals encode 16 different classes of proteins and include several plausible candidates for HvNax3. The potential of HvNax3 to provide a useful trait contributing to salinity tolerance in cultivated barley is discussed.