Label-free measurement of cell viability via counting cells attached on affinity substrates
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- 作者：Junhyoung Ahn (1)
Jina Park (1) (2)
Yeon-Gu Kim (3)
Eun Gyo Lee (3)
Min-Gon Kim (4)
Yong-Beom Shin (1) (2) - 关键词：cell viability ; label ; free measurement ; cell attachment ; CHO cell ; affinity binding
- 刊名：Biotechnology and Bioprocess Engineering
- 出版年：2014
- 出版时间：March 2014
- 年：2014
- 卷：19
- 期：2
- 页码：257-261
- 全文大小：
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Jina Park (1) (2)
Yeon-Gu Kim (3)
Eun Gyo Lee (3)
Min-Gon Kim (4)
Yong-Beom Shin (1) (2)
1. Research Center of Integrative Cellulomics, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Korea
2. Nanobiotechnology Major, University of Science and Technology, Daejeon, 305-333, Korea
3. Biotechnology Process Engineering Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Korea
4. Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju, 500-712, Korea - ISSN：1976-3816
文摘
The commonly used trypan blue dye exclusion method and other modified cell viability methods, such as fluorescein dye and tetrazolium dye exclusion, artificially introduce toxic chemicals to cells and, thus, alter cellular organelles when measuring cell viability. Therefore, cell viability could be affected by the processes currently used to observe viability. In this study, the cell viability of Chinese hamster ovary (CHO) cells was measured by simply counting attached cells after the cultured CHO cells were attached on a Concanavalin A (Con A) substrate. The efficiency of cell attachment to Con A surfaces was different for live and dead cells allowing the cell viability of CHO cells to be measured without any chemical modifications to the cells.