Diagnotic value of some Fasciola gigantica antigens

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• 作者：Said Shalaby ; Mohammad El-Bahy ; Ali Hassan ; Hatem Shalaby…
• 关键词：Antigens ; Cross reaction ; Diagnosis ; Fasciola gigantica
• 刊名：Journal of Parasitic Diseases
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：39
• 期：3
• 页码：479-486
• 全文大小：594 KB
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• 作者单位：Said Shalaby (1)
  Mohammad El-Bahy (2)
  Ali Hassan (2)
  Hatem Shalaby (3)
  Neelima Gupta (4)
  
  1. Medical Division, Department of Complementary Medicine, National Research Centre, Cairo, Egypt
  2. Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
  3. Veterinary Division, Department of Parasitology, National Research Centre, Cairo, Egypt
  4. Department of Animal Science, Centre of Excellence, MJP Rohilkhand University, Bareilly, 243006, India
  
• 刊物主题：Infectious Diseases; Health Promotion and Disease Prevention; Medicine/Public Health, general;
• 出版者：Springer India
• ISSN：0975-0703

文摘

The present study was aimed to select the specificity of antigens for Fasciola gigantica depending on its diagnostic utility and field applications. The tested antigens were coproantigen, excretory–secretory (ES) antigen and egg antigen. Coproantigen and Copro Hyperimmune serum were able to reflect the lowest level of cross-reaction with other tested F. gigantica antigens. By using SDS-PAGE, a structural homology was observed in F. gigantica ES and egg antigens. Intense cross reaction was observed between ES and egg antigens by ELISA technique even when there was no cross-reaction with coproantigen. The 27.6 kDa band proved to be the most specific in F. gigantica coproantigen and was different from the band at the same molecular weight by ES antigen. The results conclude that coproantigens show specific diagnostic ability for Fasciola and have low numbers of cross-reaction proteins reflecting its high specificity. Moreover, detection of coproantigen in faeces offers a new potential for diagnostics as compared to serum samples. This fact holds promise for a more accurate diagnostic technique in the near future for suspected Fasciola infection. Keywords Antigens Cross reaction Diagnosis Fasciola gigantica