文摘
Phospholipase D hydrolyzes phospholipids into phophatidic acid and choline, and plays important roles in growth, development, and stress response. Genome-wide identification for the PLD gene family has been made in several model species. In maize, only a few literatures are available for cloning and function elucidation of several individual members of the PLD gene family. In this study, thirteen members of the ZmPLD gene family were identified from the maize genome by homologous alignment and conserved domain analysis. Bioinformatics analysis displayed abundant diversification of the gene structure, and differential expression patterns in different development stages and organs among these members, as well as numerous cis-acting elements during their promoter sequences. The expression of these members in seedling shoot and root under abiotic stress and hormone induction was detected by quantitative reverse transcription PCR. The result showed significant differentiation of their relative expression levels in response to stress of drought, high salt, and low temperature, and to induction of abscisic acid, methyl jasmonate, and gibberellic acid. All these results suggest that structure and function of the ZmPLD gene family are diversified among the members, as well as different from the genetic model species such as Arabidopsis and rice. Laboratory experiment should be conducted to elucidate their detail functions one by one.