Cellulolytic RoboLector – towards an automated high-throughput screening platform for recombinant cellulase expression
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- 作者：Martina Mühlmann ; Martin Kunze ; Joaquim Ribeiro…
- 关键词：High ; throughput screening ; Automation ; On ; line monitoring ; Cellulase ; Microtiter plate
- 刊名：Journal of Biological Engineering
- 出版年：2017
- 出版时间：December 2017
- 年：2017
- 卷：11
- 期：1
- 全文大小：3814KB
- 刊物类别：Engineering
- 刊物主题：Biomedical Engineering; Biotechnology; Biological Techniques; Nucleic Acid Chemistry; Applied Microbiology; Environmental Engineering/Biotechnology;
- 出版者：BioMed Central
- ISSN：1754-1611
- 卷排序：11
文摘
BackgroundCellulases are key player in the hydrolyzation of cellulose. Unfortunately, this reaction is slow and a bottleneck in the process chain from biomass to intermediates and biofuels due to low activities of the enzymes. To overcome this draw back, a lot of effort is put into the area of protein engineering, to modify these enzymes by directed evolution or rational design. Huge clone libraries are constructed and have to be screened for improved variants. High-throughput screening is the method of choice to tackle this experimental effort, but up to now only a few process steps are adapted to automated platforms and little attention has been turned to the reproducibility of clone rankings.