Vascular Endothelial Growth Factor (VEGF) isoform expression and activity in human and murine lung injury

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• 作者：Andrew RL Medford (1)
  Samantha K Douglas (1)
  Sofia IH Godinho (1)
  Kay M Uppington (1)
  Lynne Armstrong (1)
  Kathleen M Gillespie (1)
  Berendine van Zyl (1)
  Terry D Tetley (2)
  Nassif BN Ibrahim (3)
  Ann B Millar (1)
  
• 刊名：Respiratory Research
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：10
• 期：1
• 全文大小：1054KB
• 参考文献：1. Ware LB, Matthay MA: The acute respiratory distress syndrome. / N Engl J Med 2000,342(18):1334鈥?349. CrossRef
  2. Carmeliet P, Ferreira V, Breier G, Pollefeyt S, Kieckens L, Gertsenstein M, Fahrig M, Vandenhoeck A, Harpal K, Eberhardt C, Declercq C, Pawling J, Moons L, Collen D, Risau W, Nagy A: Abnormal blood vessel development and lethality in embryos lacking a single VEGF allele. / Nature 1996,380(6573):435鈥?39. CrossRef
  3. Ferrara N, Gerber HP, LeCouter J: The biology of VEGF and its receptors. / Nat Med 2003, 9:669鈥?76. CrossRef
  4. Kaner RJ, Crystal RG: Compartmentalization of vascular endothelial growth factor to the epithelial surface of the human lung. / Mol Med 2001,7(4):240鈥?46.
  5. Koyama S, Sato E, Tsukadaira A, Haniuda M, Numanami H, Kurai M, Nagai S, Izumi T: Vascular endothelial growth factor mRNA and protein expression in airway epithelial cell lines in vitro. / Eur Respir J 2002,20(6):1449鈥?456. CrossRef
  6. Boussat S, Eddahibi S, Coste A, Fataccioli V, Gouge M, Housset B, Adnot S, Maitre B: Expression and regulation of vascular endothelial growth factor in human pulmonary epithelial cells. / Am J Physiol Lung Cell Mol Physiol 2000,279(2):L371-L378.
  7. Thickett DR, Armstrong L, Christie SJ, Millar AB: Vascular endothelial growth factor may contribute to increased vascular permeability in acute respiratory distress syndrome. / Am J Respir Crit Care Med 2001,164(9):1601鈥?605.
  8. Thickett DR, Armstrong L, Millar AB: A role for vascular endothelial growth factor in acute and resolving lung injury. / Am J Respir Crit Care Med 2002,166(10):1332鈥?337. CrossRef
  9. Maitre B, Boussat S, Jean D, Gouge M, Brochard L, Housset B, Adnot S, Delclaux C: Vascular endothelial growth factor synthesis in the acute phase of experimental and clinical lung injury. / Eur Respir J 2001,18(1):100鈥?06. CrossRef
  10. Hanaoka M, Droma Y, Naramoto A, Honda T, Kobayashi T, Kubo K: Vascular endothelial growth factor in patients with high-altitude pulmonary edema. / J Appl Physiol 2003, 94:1836鈥?840.
  11. Abadie Y, Bregeon F, Papazian L, Lange F, Chailley-Heu B, Thomas P, Duvaldestin P, Adnot S, Maitre B, Delclaux C: Decreased VEGF concentration in lung tissue and vascular injury during ARDS. / Eur Respir J 2005,25(1):139鈥?46. CrossRef
  12. Tischer E, Mitchell R, Hartman T, Silva M, Gospodarowicz D, Fiddes JC, Abraham JA: The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. / J Biol Chem 1991,266(18):11947鈥?1954.
  13. Robinson CJ, Stringer SE: The splice variants of vascular endothelial growth factor (VEGF) and their receptors. / J Cell Sci 2001,114(Pt 5):853鈥?65.
  14. Houck KA, Ferrara N, Winer J, Cachianes G, Li B, Leung DW: The vascular endothelial growth factor family: identification of a fourth molecular species and characterization of alternative splicing of RNA. / Mol Endocrinol 1991,5(12):1806鈥?814. CrossRef
  15. Ferrara N: Vascular endothelial growth factor: basic science and clinical progress. / Endocr Rev 2004,25(4):581鈥?11. CrossRef
  16. Fehrenbach H: Alveolar epithelial type II cell: defender of the alveolus revisited. / Respir Res 2001,2(1):33鈥?6. CrossRef
  17. Maniscalco WM, Watkins RH, Finkelstein JN, Campbell MH: Vascular endothelial growth factor mRNA increases in alveolar epithelial cells during recovery from oxygen injury. / Am J Respir Cell Mol Biol 1995,13(4):377鈥?86.
  18. Brown KR, England KM, Goss KL, Snyder JM, Acarregui MJ: VEGF induces airway epithelial cell proliferation in human fetal lung in vitro. / Am J Physiol Lung Cell Mol Physiol 2001,281(4):L1001-L1010.
  19. Roberts JR, Perkins GD, Fujisawa T, Pettigrew KA, Gao F, Ahmed A, Thickett DR: Vascular endothelial growth factor promotes physical wound repair and is anti-apoptotic in primary distal lung epithelial and A549 cells. / Crit Care Med 2007,35(9):2164鈥?170. CrossRef
  20. Raoul W, Chailley-Heu B, Barlier-Mur AM, Delacourt C, Maitre B, Bourbon JR: Effects of vascular endothelial growth factor (VEGF) on isolated fetal alveolar type II cells. / Am J Physiol Lung Cell Mol Physiol 2004,286(6):L1293-L1301. CrossRef
  21. Bernard GR, Artigas A, Brigham KL, Carlet J, Falke K, Hudson L, Lamy M, LeGall JR, Morris A, Spragg R: Report of the American-European consensus conference on ARDS: definitions, mechanisms, relevant outcomes and clinical trial coordination. The Consensus Committee. / Intensive Care Med 1994,20(3):225鈥?32. CrossRef
  22. Krafft AE, Duncan BW, Bijwaard KE, Taubenberger JK, Lichy JH: Optimization of the Isolation and Amplification of RNA From Formalin-fixed, Paraffin-embedded Tissue: The Armed Forces Institute of Pathology Experience and Literature Review. / Mol Diagn 1997,2(3):217鈥?30. CrossRef
  23. Godinho SIMA: An animal model of the acute respiratory distress syndrome (ARDS). / European Respiratory Journal 2002, 20:486s.
  24. Witherden IR, Tetley TD, Rogers DF, Donnelly LE: Isolation and culture of human alveolar type II pneumocytes. In / Methods in Molecular Medicine, Volume 56 (book). / Volume Chapter 11. Human airway inflammation; sampling techniques and analytical protocols: Humana Press Inc., Totawa, New Jersey; 2001:137鈥?46.
  25. Zhao D, Zhu BL, Ishikawa T, Quan L, Li DR, Maeda H: Real-time RT-PCR quantitative assays and postmortem degradation profiles of erythropoietin, vascular endothelial growth factor and hypoxia-inducible factor 1 alpha mRNA transcripts in forensic autopsy materials. / Legal medicine (Tokyo, Japan) 2006,8(2):132鈥?36.
  26. Koyama S, Sato E, Haniuda M, Numanami H, Nagai S, Izumi T: Decreased level of vascular endothelial growth factor in bronchoalveolar lavage fluid of normal smokers and patients with pulmonary fibrosis. / Am J Respir Crit Care Med 2002,166(3):382鈥?85. CrossRef
  27. Lieto E, Ferraraccio F, Orditura M, Castellano P, Mura AL, Pinto M, Zamboli A, De Vita F, Galizia G: Expression of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) is an independent prognostic indicator of worse outcome in gastric cancer patients. / Annals of Surgical Oncology 2008,15(1):69鈥?9. CrossRef
  28. Mura M, Dos Santos CC, Stewart D, Liu M: Vascular endothelial growth factor and related molecules in acute lung injury. / J Appl Physiol 2004,97(5):1605鈥?617. CrossRef
  29. Mura M, Han B, Andrade CF, Seth R, Hwang D, Waddell TK, Keshavjee S, Liu M: The early responses of VEGF and its receptors during acute lung injury: implication of VEGF in alveolar epithelial cell survival. / Critical care (London, England) 2006,10(5):R130. CrossRef
  30. Perkins GD, Roberts J, McAuley DF, Armstrong L, Millar A, Gao F, Thickett DR: Regulation of vascular endothelial growth factor bioactivity in patients with acute lung injury. / Thorax 2005,60(2):153鈥?58. CrossRef
  31. Shraga-Heled N, Kessler O, Prahst C, Kroll J, Augustin H, Neufeld G: Neuropilin-1 and neuropilin-2 enhance VEGF121 stimulated signal transduction by the VEGFR-2 receptor. / Faseb J 2007,21(3):915鈥?26. CrossRef
  32. Montgomery AB, Stager MA, Carrico CJ, Hudson LD: Causes of mortality in patients with the adult respiratory distress syndrome. / Am Rev Respir Dis 1985,132(3):485鈥?89.
  33. Medford AR, Millar AB: Vascular endothelial growth factor (VEGF) in acute lung injury (ALI) and acute respiratory distress syndrome (ARDS): paradox or paradigm? / Thorax 2006,61(7):621鈥?26. CrossRef
  34. Berse B, Brown LF, Van de WL, Dvorak HF, Senger DR: Vascular permeability factor (vascular endothelial growth factor) gene is expressed differentially in normal tissues, macrophages, and tumors. / Mol Biol Cell 1992,3(2):211鈥?20.
  35. Corne J, Chupp G, Lee CG, Homer RJ, Zhu Z, Chen Q, Ma B, Du Y, Roux F, McArdle J, Waxman AB, Elias JA: IL-13 stimulates vascular endothelial cell growth factor and protects against hyperoxic acute lung injury. / J Clin Invest 2000,106(6):783鈥?91. CrossRef
  36. Compernolle V, Brusselmans K, Acker T, Hoet P, Tjwa M, Beck H, Plaisance S, Dor Y, Keshet E, Lupu F, Nemery B, Dewerchin M, Van Veldhoven P, Plate K, Moons L, Collen D, Carmeliet P: Loss of HIF-2alpha and inhibition of VEGF impair fetal lung maturation, whereas treatment with VEGF prevents fatal respiratory distress in premature mice. / Nat Med 2002,8(7):702鈥?10.
  37. Karmpaliotis D, Kosmidou I, Ingenito EP, Hong K, Malhotra A, Sunday ME, Haley KJ: Angiogenic growth factors in the pathophysiology of a murine model of acute lung injury. / Am J Physiol Lung Cell Mol Physiol 2002,283(3):L585-L595.
  38. Watkins RH, D'Angio CT, Ryan RM, Patel A, Maniscalco WM: Differential expression of VEGF mRNA splice variants in newborn and adult hyperoxic lung injury. / Am J Physiol 1999,276(5 Pt 1):L858-L867.
  39. Berthiaume Y, Lesur O, Dagenais A: Treatment of adult respiratory distress syndrome: plea for rescue therapy of the alveolar epithelium. / Thorax 1999,54(2):150鈥?60. CrossRef
  40. Ohwada A, Yoshioka Y, Iwabuchi K, Nagaoka I, Dambara T, Fukuchi Y: VEGF regulates the proliferation of acid-exposed alveolar lining epithelial cells. / Thorax 2003,58(4):328鈥?32. CrossRef
  
• 作者单位：Andrew RL Medford (1)
  Samantha K Douglas (1)
  Sofia IH Godinho (1)
  Kay M Uppington (1)
  Lynne Armstrong (1)
  Kathleen M Gillespie (1)
  Berendine van Zyl (1)
  Terry D Tetley (2)
  Nassif BN Ibrahim (3)
  Ann B Millar (1)
  
  1. Department of Clinical Science at North Bristol, University of Bristol Paul O'Gorman Lifeline Centre, Southmead Hospital, Westbury-on-Trym, BS10 5NB, Bristol, UK
  2. Lung Cell Biology, National Heart & Lung Institute, Imperial College, Dovehouse Street, SW3 6LY, London, UK
  3. Department of Pathology, North Bristol NHS Trust, Frenchay Hospital, Frenchay Park Road, Frenchay, BS16 1LE, Bristol, UK
  

文摘

Background The properties of vascular endothelial growth factor (VEGF) as a potent vascular permogen and mitogen have led to investigation of its potential role in lung injury. Alternate spliced VEGF transcript generates several isoforms with potentially differing functions. The purpose of this study was to determine VEGF isoform expression and source in normal and ARDS subjects and investigate the expression and regulation of VEGF isoforms by human alveolar type 2 (ATII) cells. Methods VEGF protein expression was assessed immunohistochemically in archival normal and ARDS human lung tissue. VEGF isoform mRNA expression was assessed in human and murine lung tissue. Purified ATII cells were cultured with proinflammatory cytokines prior to RNA extraction/cell supernatant sampling/proliferation assay. Measurements and Main Results VEGF was expressed on alveolar epithelium, vascular endothelium and alveolar macrophages in normal and ARDS human lung tissue. Increases in VEGF expression were detected in later ARDS in comparison to both normal subjects and early ARDS (p < 0.001). VEGF121, VEGF165 and VEGF189 isoform mRNA expression increased in later ARDS (p < 0.05). The ratio of soluble to cell-associated isoforms was lower in early ARDS than normal subjects and later ARDS and also in murine lung injury. ATII cells constitutionally produced VEGF165 and VEGF121 protein which was increased by LPS (p < 0.05). VEGF165 upregulated ATII cell proliferation (p < 0.001) that was inhibited by soluble VEGF receptor 1 (sflt) (p < 0.05). Conclusion These data demonstrate that changes in VEGF isoform expression occur in ARDS which may be related to their production by and mitogenic effect on ATII cells; with potentially significant clinical consequences.