The effect of shock wave therapy on gene expression in human osteoblasts isolated from hypertrophic fracture non-unions

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• 作者：A. Hofmann ; U. Ritz ; J.-D. Rompe ; A. Tresch ; P. M. Rommens
• 关键词：Extracorporeal shock wave therapy ; Fracture non ; union ; Osteoblast ; Gene expression ; Microarray
• 刊名：Shock Waves
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：25
• 期：1
• 页码：91-102
• 全文大小：395 KB
• 参考文献：1. Rompe, J.D., Rosendahl, T., Schollner, C., Theis, C.: High-energy extracorporeal shock wave treatment of nonunions. Clin. Orthop. 387, 102-11 (2001) CrossRef
  2. Wang, F.S., Yang, K.D., Chen, R.F., Wang, C.J., Sheen-Chen, S.M.: Extracorporeal shock wave promotes growth and differentiation of bone-marrow stromal cells towards osteoprogenitors associated with induction of TGF-beta1. J. Bone Joint Surg. Br. 84(3), 457-61 (2002) CrossRef
  3. Chooi, Y.S., Penafort, R.: Extra-corporeal shock-wave therapy in the treatment of non-unions. Med. J. Malaysia 59(5), 674-77 (2004)
  4. Maier, M., Milz, S., Tischer, T., Munzing, W., Manthey, N., Stabler, A., Holzknecht, N., Weiler, C., Nerlich, A., Refior, H.J., Schmitz, C.: Influence of extracorporeal shock-wave application on normal bone in an animal model in vivo. Scintigraphy, MRI and histopathology. J. Bone Joint Surg. Br. 84(4), 592-99 (2002) CrossRef
  5. Tam, K.F., Cheung, W.H., Lee, K.M., Qin, L., Leung, K.S.: Delayed stimulatory effect of low-intensity shockwaves on human periosteal cells. Clin. Orthop. Relat Res. 438(260-), 260-65 (2005) CrossRef
  6. Martini, L., Fini, M., Giavaresi, G., Torricelli, P., de Pretto, M., Rimondini, L., Giardino, R.: Primary osteoblasts response to shock wave therapy using different parameters. Artif. Cells Blood Substit. Immobil. Biotechnol. 31(4), 449-66 (2003) CrossRef
  7. Martini, L., Giavaresi, G., Fini, M., Torricelli, P., de Pretto, M., Schaden, W., Giardino, R.: Effect of extracorporeal shock wave therapy on osteoblastlike cells. Clin. Orthop. 413, 269-80 (2003) CrossRef
  8. Megas, P.: Classification of non-union. Injury. 36 Suppl 4:S30-, S30–S37 (2005)
  9. Johannes, E.J., Kaulesar Sukul, D.M., Matura, E.: High-energy shock waves for the treatment of nonunions: an experiment on dogs. J. Surg. Res. 57(2), 246-52 (1994) CrossRef
  10. Hofmann, A., Hessmann, M.H., Meurer, A., Rommens, P.M., Heine, J., Rompe, J.D.: Extracorporeal shock wave induced changes in function of osteoblast-like cells. Proceedings of American Academy of Orthopaedic Surgeons (AAOS), 613-13 (2005)
  11. Wang, F.S., Wang, C.J., Sheen-Chen, S.M., Kuo, Y.R., Chen, R.F., Yang, K.D.: Superoxide mediates shock wave induction of ERK-dependent osteogenic transcription factor (CBFA1) and mesenchymal cell differentiation toward osteoprogenitors. J. Biol. Chem. 277(13), 10931-0937 (2002) CrossRef
  12. Rompe, J.D., Decking, J., Schoellner, C., Nafe, B.: Shock wave application for chronic plantar fasciitis in running athletes. A prospective, randomized, placebo-controlled trial. Am. J. Sports Med. 31(2), 268-75 (2003)
  13. U.S. Food and Drug Administration, C.f.D.a.R.H.: Extracorporeal Shock Wave Therapy (ESWT) system - Approval letter. Application Number P010039. Summary of Safety and Effectiveness. Retrieved from www.accessdata.fda.gov/scripts/cdrh/cfdocs/cftopic/pma/pma.cfm?num=p010039 (2002)
  14. Shih, J.H., Michalowska, A.M., Dobbin, K., Ye, Y.M., Qiu, T.H., Green, J.E.: Effects of pooling mRNA in microarray class comparisons. Bioinformatics 20(18), 3318-325 (2004) CrossRef
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• 作者单位：A. Hofmann (1)
  U. Ritz (1)
  J.-D. Rompe (2)
  A. Tresch (3)
  P. M. Rommens (1)
  
  1. Department for Orthopedics and Traumatology, University Medical Center, Langenbeckstr. 1, 55101, Mainz, Germany
  2. OrthoTrauma Evaluation Center, Mainz, Germany
  3. Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, Cologne, Germany
  
• 刊物类别：Physics and Astronomy
• 刊物主题：Physics
  Mechanics, Fluids and Thermodynamics
  Fluids
  Thermodynamics
  Acoustics
  Condensed Matter
  Solid State Physics and Spectroscopy
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-2153

文摘

Shock wave therapy has been increasingly evaluated as a non-invasive alternative for the treatment of delayed fracture healing and non-unions. Although several clinical studies showed a beneficial effect especially for the hypertrophic type of non-union, little is known about the biological mechanism of its osteogenic effect. To identify the molecular background for the positive effect of shock waves on healing of fracture non-unions, we have analyzed the changes of the global gene expression in human osteoblasts after exposure to shock waves of different energy flux densities. Human osteoblasts were isolated from five patients at non-union sites, treated with 500 impulses of energy flux densities of 0.06 and \(0.5\,{\text {mJ/mm}}^{2}\) , and cultured for 96?h. \(\text {Affymetrix}^\circledR \) HG-U133A microarrays were used for the analysis of the shock wave-regulated mRNA-transcripts. Differential gene expression was verified by reverse transcriptase polymerase chain reactions. We identified 47 transcripts that showed differential expression after \(0.06\,{\text {mJ/mm}}^{2}\) and 45 transcripts after \(0.5\,{\text {mJ/mm}}^{2}\) energy treatment. Most intriguing was the up-regulation of neprilysin, calmegin, osteoglycin, asporin, and interleukin-13 receptor- \(\upalpha 2\) . Eighteen identified genes were previously described to fulfill an important function in bone growth and metabolism. Our study provides the first molecular profile of shock wave-induced gene expression changes in human osteoblasts from patients with hypertrophic fracture non-unions, and it offers a possible molecular explanation for the positive effects of shock waves in patients ridden with this disease .