Pathophysiological Scenario of Hematopoietic Disorders: A Comparative Study of Aplastic Anemia, Myelodysplastic Syndrome and Leukemia in Experimental Animals

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• 作者：Ritam Chatterjee ; Sukalpa Chattopadhyay…
• 关键词：Hematopoiesis ; Aplastic anemia ; MDS ; Leukemia ; Pathophysiology
• 刊名：Proceedings of the Zoological Society
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：69
• 期：1
• 页码：114-124
• 全文大小：2,003 KB
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• 作者单位：Ritam Chatterjee (1)
  Sukalpa Chattopadhyay (1)
  Shalini Sanyal (1)
  Suchismita Daw (1)
  Sujata Law (2)
  
  1. Stem Cell Research and Application Unit, Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, C R Avenue, Kolkata, 700073, India
  2. Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, 700073, India
  
• 刊物主题：Life Sciences, general; Zoology; Animal Anatomy / Morphology / Histology; Animal Genetics and Genomics; Biodiversity; Conservation Biology/Ecology;
• 出版者：Springer India
• ISSN：0974-6919

文摘

The conversion of physiology to pathophysiology in hematological disorders viz: aplastic anemia, myelodysplastic syndrome (MDS) and leukemia in murine models was the subject of study in the present programme. Peripheral blood hemogram, spleno-somatic index, bone marrow smear study, cytochemical staining of marrow, cell release kinetics study during marrow explants culture, hematopoietic niche assessment, chromosomal aberration study, plasma membrane stability study of marrow cells, lysosomal membrane and mitochondrial membrane stability study and innate immune parameters were performed in the aplastic anemia, leukemia and MDS mouse model. In bone marrow aplasia, peripheral blood pancytopenia, marrow hypocellularity, decreased marrow cellular viability, deterioration of bone marrow hematopoiesis as well as hematopoietic microenvironment and extramedullary hematopoiesis were noticed. In addition, disruption of mitochondrial and lysosomal membrane integrity along with reduction of innate immune parameters were found in the hematopoietic suppressed condition. Surprisingly, no noticeable chromosomal aberration was found in the aplastic condition. Ineffective marrow hematopoiesis together with the disruption of hematopoietic microenvironment was observed in MDS. Also, extramedullary hematopoiesis, increased marrow cellular death, chromosomal aberration and loss of innate immunity were the common events. During leukemia, the number of functionally and structurally immature cells in the peripheral blood and bone marrow was increased together with malignant conversion of hematopoietic cells in the presence of malignancy supportive stromal microenvironment. Chromosomal aberration, decrease of cell mediated immunity with least mitochondrial apoptotic damage were also found in leukemic condition as well.