A novel link between angiogenesis and natural products: Anti-angiogenic effects of Opuntia dillenii

详细信息    查看全文

• 作者：Sohail Ejaz (1)
  Khaleeq Anwar (2)
  Robina Taj (2)
  Muhammad Ashraf (2)
  
• 关键词：Angiogenesis ; Opuntia dillenii ; CAM assay ; Phyto chemicals
• 刊名：Central European Journal of Biology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：9
• 期：3
• 页码：298-308
• 全文大小：
• 作者单位：Sohail Ejaz (1)
  Khaleeq Anwar (2)
  Robina Taj (2)
  Muhammad Ashraf (2)
  
  1. Department of Clinical Neurosciences, Stroke Research Group, Addenbrooke’s Hospital, University of Cambridge, Cambridge, CB2 2QQ, UK
  2. Angiogenesis and Toxicology Research Laboratory, Department of Pharmacology and Toxicology, University of Veterinary and Animal Sciences, 54000, Lahore, Pakistan
  
• ISSN：1644-3632

文摘

Angiogenesis is the formation of new blood vessels from the existing blood vessels and is involved in both physiological and pathological events. Pathological angiogenesis provokes in several important diseases as in inflammatory diseases, ischemic conditions, diabetic neuropathy and cancer. The discovery of new drugs from phyto-chemicals has a long history and anti-angiogenic agents from plant sources provide a platform for the development of phyto-medicines to treat/control pathological angiogenesis. The current project was designed to determine the efficacy of different concentrations of aqueous extract from Opuntia dillenii (OD) on angiogenesis by employing chicken chorioallantoic membrane (CAM) assay. Computer based 3D image probing was utilized to quantify anti-angiogenic effects of OD extract. Extremely aggravated dose dependent anti-angiogenic response was recorded in all groups treated with OD extract with significant reduction (P<0.01) in total area, diameter of the primary, secondary, tertiary blood vessels and capillary plexuses. Analysis of 3D parameters of OD treated CAMs revealed deteriorated angular spectrum and reduction in the height of Abbott curves. In addition, histological data revealed thinning of ectodermal layer and damaged extracellular matrix. The results spotlight that OD extract possess considerable anti-angiogenic potential and further characterization/isolation of diverse phyto-chemicals would turn up with the discovery of novel phyto-agents to control/mitigate pathological angiogenesis.