Increased gut microbiota diversity and abundance of Faecalibacterium prausnitzii and Akkermansia after fasting: a pilot study

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• 作者：Marlene Remely ; Berit Hippe ; Isabella Geretschlaeger…
• 关键词：Akkermansia ; Fasting ; Laxative ; Probiotic ; Microbial diversity
• 刊名：Wiener klinische Wochenschrift
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：127
• 期：9-10
• 页码：394-398
• 全文大小：362 KB
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• 作者单位：Marlene Remely (1)
  Berit Hippe (1)
  Isabella Geretschlaeger (1)
  Sonja Stegmayer (1)
  Ingrid Hoefinger (2)
  Alexander Haslberger (1)
  
  1. Institute of Nutritional Sciences, University Vienna, Althahnstra脽e 14, 1090, Vienna, Austria
  2. Essen & Fasten by Ingrid Hoefinger, Purkersdorf, Austria
  
• 刊物主题：Medicine/Public Health, general; Internal Medicine; Endocrinology; Gastroenterology; Pneumology/Respiratory System;
• 出版者：Springer Vienna
• ISSN：1613-7671

文摘

Summary Background An impaired gut microbiota has been reported as an important factor in the pathogenesis of obesity. Weight reduction has already been mentioned to improve gut microbial subpopulations involved in inflammatory processes, though other subpopulations still need further investigation. Thus, weight reduction in the context of a fasting program together with a probiotic intervention may improve the abundance and diversity of gut microbiota. Methods In this pilot study, overweight people underwent a fasting program with laxative treatment for 1 week followed by a 6 week intervention with a probiotic formula. Gut microbiota were analyzed on the basis of 16s rDNA with a quantitative real time polymerase chain reaction. Additionally, a food frequency questionnaire with questions about nutritional behavior, lifestyle, and physical activity was administered before and after the intervention. Results We observed an increase in microbial diversity over the study period. No significant changes in abundance of total bacteria, or of Bacteroidetes, Prevotella, Clostridium cluster XIVa, or Clostridium cluster IV were found, although Faecalibacterium prausnitzii showed an increase over the study period. In addition, Akkermanisa and Bifidobacteria increased in abundance due to intervention. The inflammation-associated gut microbes Enterobacteria and Lactobacilli increased during the first week and then declined by the end of the intervention. Two-thirds of the study participants harbored Archaea. No significant improvements of eating habits were reported, although physical activity improved due to the intervention. Conclusions Our results show that caloric restriction affects gut microbiota by proliferating mucin-degrading microbial subpopulations. An additional intervention with a probiotic formula increased probiotic-administered gut microbial populations.