Expression of bone morphogenetic protein 2, 4, and related components of the BMP signaling pathway in the mouse uterus during the estrous cycle

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• 作者：Yan Li (1)
  Quan-wei Wei (1)
  Jian-gang Feng (1)
  Mu-lin Xu (1)
  Rui-hua Huang (1)
  Fang-xiong Shi (1)
  
• 关键词：Bone morphogenetic protein (BMP) ; BMP receptor ; SMAD ; Uterus ; Estrous cycle ; Q492.5 ; /li> /li> SMAD ; ;
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2014
• 出版时间：July 2014
• 年：2014
• 卷：15
• 期：7
• 页码：601-610
• 全文大小：760 KB
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• 作者单位：Yan Li (1)
  Quan-wei Wei (1)
  Jian-gang Feng (1)
  Mu-lin Xu (1)
  Rui-hua Huang (1)
  Fang-xiong Shi (1)
  
  1. College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, 210095, China
  
• ISSN：1862-1783

文摘

The objective was to investigate the expression of bone morphogenetic protein (BMP) family members in the mouse uterus during the estrous cycle by real-time polymerase chain reaction (PCR) and immunohistochemistry. Uterine samples from Swiss ICR mice were collected and dissected free of surrounding tissue. One uterine horn was snap frozen in liquid nitrogen immediately after collection and stored at 0 °C for RNA extraction, and the other was fixed in 40 mg/ml paraformaldehyde at room temperature for immunolocalization of BMP2 protein. Real-time PCR analysis showed that the expression level of Bmp2 was significantly higher at proestrus than at estrus and metestrus (PBmp4 exhibited significant fluctuations, but there were no statistically significant differences between the expression levels of Bmp2 and Bmp4 (P>0.05). The expression levels of Bmpr1a and Bmpr2 remained unchanged during estrous cycles. However, the level of Bmpr1b mRNA decreased significantly at estrus (PBmpr1b mRNA was significantly lower than those of Bmpr1a and Bmpr2 mRNA at the corresponding stages (PR-Smads) detected were differentially expressed in the mouse uterus and the expression levels of Smad1 and Smad5 were significantly higher than that of Smad8 (PSmad4 did not change substantially throughout the estrous cycle. Immunohistochemical experiments revealed that BMP2 protein was differentially expressed and localized mainly in the uterine luminal and glandular epithelial cells throughout the estrous cycle. In conclusion, our results provide information about the variation in the mRNA levels of Bmp2 and Bmp4 and related components of the BMP signaling pathway. The data provide quantitative and useful information about the roles of endometrial BMP proposed and demonstrated by others, such as the degradation and remodeling of the endometrium.