Selecting DNA aptamers for endotoxin separation

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• 作者：GuoQing Ying ; FangFang Zhu ; Yu Yi ; JianShu Chen ; JianFeng Mei…
• 关键词：Aptamer ; Endotoxin ; Magnetic beads ; Systematic evolution of ligands by exponential enrichment (SELEX) ; Separation
• 刊名：Biotechnology Letters
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：37
• 期：8
• 页码：1601-1605
• 全文大小：573 KB
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• 作者单位：GuoQing Ying (1) (2)
  FangFang Zhu (2)
  Yu Yi (2)
  JianShu Chen (2)
  JianFeng Mei (2)
  YanLu Zhang (2)
  ShuQing Chen (1)
  
  1. College of Pharmaceutical Science, ZheJiang University, Hangzhou, 310013, China
  2. College of Pharmaceutical Science, ZheJiang University of Technology, Hangzhou, 310014, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  Applied Microbiology
  Biochemistry
  
• 出版者：Springer Netherlands
• ISSN：1573-6776

文摘

Objectives To select aptamers for endotoxin separation from a 75-nucleotide single-stranded DNA random library using systematic evolution of ligands by exponential enrichment. Results After 15 rounds of selection, the final pool of aptamers was specific to endotoxin. Structural analysis of aptamers that appeared more than once suggested that one aptamer can form a G-quartet structure. Tests for binding affinity and specificity showed that this aptamer exhibited a high affinity for endotoxin. Using this aptamer, aptamer-magnetic beads were designed to separate endotoxin. Conclusions Using these aptamer-magnetic beads, a new method to separate endotoxin was developed to enable specific separation of endotoxin that can be applied to drug and food products.