Candida clinical species identification: molecular and biochemical methods

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• 作者：Ana Rita Costa (1)
  Filipe Silva (2)
  Mariana Henriques (1)
  Joana Azeredo (1)
  Rosário Oliveira (1)
  Alberta Faustino (1)
  
• 关键词：Candida species ; Discriminating potential ; PCR ; Vitek 2 YST ; Auxacolor ; ID32C
• 刊名：Annals of Microbiology
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：60
• 期：1
• 页码：105-112
• 全文大小：170KB
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• 作者单位：Ana Rita Costa (1)
  Filipe Silva (2)
  Mariana Henriques (1)
  Joana Azeredo (1)
  Rosário Oliveira (1)
  Alberta Faustino (1)
  
  1. Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, Universidade do Minho, 4710-057, Braga, Portugal
  2. Hospital de S. Marcos, Largo Carlos Amarante, 4701-965, Braga, Portugal
  
• ISSN：1869-2044

文摘

In the last decade, the number and diversity of nosocomial Candida infections has increased significantly, resulting in an emergent need for rapid and accurate methods for Candida identification. Therefore, the aim of this study was to evaluate the performance of three biochemical systems (Auxacolor, ID32C, and Vitek 2 YST) for the identification of Candida species, comparing them with molecular identification (polymerase chain reaction and gel agarose electrophoresis). These methods were used to assess Candida spp. (229 clinical isolates) prevalence and distribution among clinical specimens. The biochemical methods with higher percentages of correct identification were Vitek 2 YST (79.6%) and Auxacolor (78.6%). However, overall the biochemical methods assayed differed from the molecular identification. Thus, due to their rapid and precise identification, molecular methods are promising techniques for Candida species identification in clinical laboratories. Candida albicans and Non Candida albicans Candida species had a similar prevalence (50.4 and 49.6%, respectively), corroborating the epidemiological shift observed for these pathogens in the recent years.