Production of Thermostable Lipase by Thermomyces lanuginosus on Solid-State Fermentation: Selective Hydrolysis of Sardine Oil

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• 作者：Nayeli ávila-Cisneros (1)
  Susana Velasco-Lozano (1)
  Sergio Huerta-Ochoa (1)
  Jesús Córdova-López (2)
  Miquel Gimeno (3)
  Ernesto Favela-Torres (1) (4)
  
• 关键词：Solid ; state fermentation ; Thermomyces lanuginosus ; Thermostable lipases ; Sardine oil hydrolysis
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：174
• 期：5
• 页码：1859-1872
• 全文大小：355 KB
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• 作者单位：Nayeli ávila-Cisneros (1)
  Susana Velasco-Lozano (1)
  Sergio Huerta-Ochoa (1)
  Jesús Córdova-López (2)
  Miquel Gimeno (3)
  Ernesto Favela-Torres (1) (4)
  
  1. Universidad Autónoma Metropolitana—Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, Mexico, 09340, D.F., Mexico
  2. Departamento de Química, Universidad de Guadalajara, Guadalajara, Mexico
  3. Facultad de Química, Departamento de Alimentos y Biotecnología, Universidad Nacional Autónoma de México, Ciudad Universitaria, Mexico, D.F., 04510, Mexico
  4. Departamento de Biotecnología, Universidad Autónoma Metropolitana—Unidad Iztapalapa, Av. San Rafael Atlixco 186, Col. Vicentina, Iztapalapa, Mexico, 09340, D.F., Mexico
  
• ISSN：1559-0291

文摘

A naturally immobilized biocatalyst with lipase activity was produced by Thermomyces lanuginosus on solid-state fermentation with perlite as inert support. Maxima lipase activities (22 and 120?U/g of dry matter, using p-nitrophenyl octanoate and trioctanoine, respectively, as substrates) were obtained after 72?h of solid culture, remaining nearly constant up to 120?h. Maxima lipase activity was found at 60 to 85?°C and pH 10. The biocatalyst was stable at 60?°C for at least 4?h of incubation and a pH from 7 to 10. Energy values of activation and deactivation of lipase were of 26 and 6.7?kJ/mol, respectively. The biocatalyst shows high selectivity for the release of the omega-3 polyunsaturated fatty acids, eicosapentaenoic (EPA) and docosahexaenoic acids (DHA), during the hydrolysis of sardine oil. The EPA/DHA ratio (16:6) released by this biocatalyst was superior to that obtained with the commercial preparations of T. lanuginosus.