Feather degradation potential of Stenotrophomonas maltophilia KB13 and feather protein hydrolysate (FPH) mediated reduction of hexavalent chromium

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• 作者：Khushboo Bhange ; Venkatesh Chaturvedi ; Renu Bhatt
• 关键词：Stenotrophomonas maltophilia KB13 ; Feather degradation ; Feather protein hydrolysate ; Cr(VI) reduction
• 刊名：3 Biotech
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：1
• 全文大小：760 KB
• 参考文献：Adari KK, Nowduri A, Parvataneni V (2008) Oxidation of l -cys by chromium(VI)—a kinetic study. Bull Chem Soc 22:305–310
  Alam MZ, Ahmad S (2012) Toxic chromate reduction by resistant and sensitive bacteria isolated from tannery effluent contaminated soil. Ann Microbiol 62:113–121CrossRef
  Alam MZ, Malik A (2008) Chromate resistance, transport and bioreduction by Exiguobacterium sp. ZM-2 isolated from agricultural soil irrigated with tannery effluent. J Basic Microbiol 48:416–420CrossRef
  Allegretti P, Furlong J, Donati E (2006) The role of higher polythionates in the reduction of chromium(VI) by Acidithiobacillus and Thiobacillus cultures. J Biotechnol 122:55–61CrossRef
  Anbu P, Gopinath SCB, Hilda A, Lakshmipriya T, Annadurai G (2007) Optimization of extracellular keratinase production by poultry farm isolates Scopulariopsis brevicaulis. Bioresour Technol 98:1298–1303CrossRef
  Ataabadi M, Hoodaji M, Tahmourespour A, Kalbasi M, Abdouss M (2015) Optimization of factors affecting hexavalent chromium removal from simulated electroplating wastewater by synthesized magnetite nanoparticles. Environ Monit Assess 187:4165CrossRef
  Bach E, Anna VS, Daroit DJ, Correa AP, Segalin J, Brandelli A (2012) Production, one-step purification, and characterization of keratinolytic protease from Serratia marcescens P3. Process Biochem 47:2455–2462
  Bálint B, Bagi Z, Tóth A, Rákhely G, Perei K, Kovács K (2005) Utilization of keratin-containing biowaste to produce biohydrogen. Appl Microbiol Biotechnol 69:404–410CrossRef
  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  Chaturvedi V, Bhange K, Bhatt R, Verma P (2014) Production of keratinase using chicken feathers s substrate by a novel multifunctional strain of Pseudomonas stutzeri and its dehairing application. Biocatal Agric Biotechnol 3:167–174
  Chen ZL, Megharaj M, Naidu R (2007) Speciation of chromium in waste water using ion chromatography inductively coupled plasma mass spectrometry. Talanta 72:394–400CrossRef
  Cheng X, Huang L, Tu X, Li K (2010) Medium optimization for the feather degradation by Streptomyces fradiae Var S-221 using the response surface methodology. Biodegradation 21:117–122CrossRef
  De Logu A, Uda P, Pellerano ML, Pouseda ML (2001) Comparison of two rapid calorimetric methods for determining resistance of Mycobacterium tuberculosis to rifampin, isoniazid, streptomycin in liquid medium. Eur J Clin Microbiol 20:33–39CrossRef
  Deng S, Yu Q, Huang J, Yu G (2010) Removal of perfluorooctane sulfonate from wastewater by anion exchange resins: effects of resin properties and solution chemistry. Water Res 44:5188–5195CrossRef
  Eaton AD, Clesceri LS, Rice EW, Greenberg AE, Franson MAH (2005) Standard methods for the examination of water and wastewater: Centennial edition. American Public Health Association, Washington DC, p 1368
  Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77CrossRef
  Fang Z, Zhang J, Liu B, Du G, Chen J (2013) Biodegradation of wool waste and keratinase production in scale-up fermenter with different strategies by Stenotrophomonas maltophilia BBE11-1. Bioresour Technol 140:286–291CrossRef
  Focardi S, Pepi M, Landi G, Gasperini S, Ruta M, Biasio PD, Focardi SE (2012) Hexavalent chromium reduction by whole cells and cell free extract of the moderate halophilic bacterial strain Halomonas sp. TA-04. Int Biodeterior Biodegrad 66:63–70CrossRef
  Fontoura R, Daroit DJ, Correa AP, Meira SM, Mosquera M, Brandelli A (2005) Production of feather hydrolysates with antioxidant, angiotensin-I converting enzyme and dipeptidyl peptidase-IV-inhibitory activities. New Biotechnol 31:506–513CrossRef
  Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Lippincott Williams and Wilkins, Philadelphia
  Jeong JH, Lee OM, Jeon YD, Kim JD, Lee NR, Lee CY, Son HJ (2010) Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity. Process Biochem 45:1738–1745CrossRef
  Kwong DWJ, Pennington DE (1983) Stoichiometry, kinetics, and mechanisms of the chromium(V1) oxidation of l -cysteine at neutral pH. Inorg Chem 23:2532–2537
  Lasekan A, Abu Bakar F, Hashim D (2013) Potential of chicken by-products as sources of useful biological resources. Waste Manage 33:552–565CrossRef
  Lateef A, Oloke JK, Gueguim EB, Sobowale BO, Ajao SO, Bello BY (2010) Keratinolytic activities of a new feather-degrading isolate of Bacillus cereus LAU 08 isolated from Nigerian soil. Int Biodeterior Biodegrad 64:162–165CrossRef
  Lo W, Too J, Wu JY (2012) Production of keratinolytic enzyme by an indigenous feather-degrading strain Bacillus cereus Wu2. J Biosci Bioeng 114:1–8CrossRef
  Megharaj M, Avudainayagam S, Naidu R (2003) Toxicity of hexavalent chromium and its reduction by bacteria isolated from soil contaminated with tannery waste. Curr Microbiol 47:51–54CrossRef
  Quievryn G, Goulart M, Messer J, Zhitkovich A (2001) Reduction of Cr(VI) by cysteine: significance in human lymphocytes and formation of DNA damage in reactions with variable reduction rates. Mol Cell Biochem 222:107–118CrossRef
  Ren T, He P, Niu W, Wu Y, Ai L, Gou X (2013) Synthesis of α-Fe2O3 nanofibers for applications in removal and recovery of Cr(VI) from wastewater. Environ Sci Pollut R 20:155–162CrossRef
  Riffel A, Brandelli A, Bellato CM, Souza GHMF, Eberlin MN, Tavares FCA (2007) Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. kr6. J Biotechnol 128:693–703CrossRef
  Santhana KKA, Kumar CU, Rajesh V, Rajesh N (2014) Microwave assisted preparation of n-butylacrylate grafted chitosan and its application for Cr(VI) adsorption. Int J Biol Macromol 66:135–143CrossRef
  Shrinivas D, Naik GR (2011) Characterization of alkaline thermostable keratinolytic protease from thermoalkylophilic Bacillus halodurans JB99 exhibiting dehairing activity. Int Biodeterior Biodegrad 65:29–35CrossRef
  Sultan S, Hasnain S (2007) Reduction of toxic hexavalent chromium by Ochrobacterium intermedium strain SDCr-5 stimulated by heavy metals. Bioresour Technol 98:340–344CrossRef
  Syed DG, Lee JC, Li WJ, Kim CJ, Agasar D (2009) Production characterization and application of keratinase from Streptomyces gulbargensis. Bioresour Technol 100:1868–1871
  Taskin M (2013) A new strategy for improved glutathione production from Saccharomyces cerevisiae: use of cysteine- and glycine-rich chicken feather protein hydrolysate as a new cheap substrate. J Sci Food Agric 93:535–541CrossRef
  Taskin M, Ozkan B, Atici O, Aydogan MN (2012) Utilization of chicken feather hydrolysate as a novel fermentation substrate for production of exopolysaccharide and mycelial biomass from edible mushroom Morchella esculenta. Int J Food Sci Nutr 63:597–602CrossRef
  Tatineni R, Doddapaneni KK, Potumarthi RC, Vellanki RN, Kandathil MT, Kolli N, Mangamoori LN (2008) Purification and characterization of an alkaline keratinase from Streptomyces Sp. Bioresour Technol 99:1596–1602CrossRef
  Thatoi H, Das S, Mishra J, Rath BP, Das N (2014) Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review. J Environ Manage 146:383–399CrossRef
  Tiwary E, Gupta R (2010) Medium optimization for a novel 58 kDa dimeric keratinase from Bacillus licheniformis ER-15; Biochemical characterization and application in feather degradation and dehairing of hides. Bioresour Technol 101:6103–6110CrossRef
  Tsopmo A, Gao Q, Baakdah MM (2014) Reduction of hexavalent chromium by digested oat bran proteins. Food Chem 153:171–176CrossRef
  Wang WQ, Li MY, Zeng QX (2012) Thermodynamics of Cr(VI) adsorption on strong alkaline anion exchange fiber. Trans Nonferrous Metals Soc 22:2831–2839CrossRef
  Welch CM, Nekrassova O, Compton RG (2005) Reduction of hexavalent chromium at solid electrodes in acidic media: reaction mechanism and analytical applications. Talenta 65:74–80
  Yamamura S, Morita Y, Hasan Q, Yokoyama K, Tamiya E (2002) Keratin degradation: a cooperative action of two enzymes from Stenotrophomonas sp. Biochem Biophys Res Commun 294:1138–1143CrossRef
  
• 作者单位：Khushboo Bhange (1)
  Venkatesh Chaturvedi (2)
  Renu Bhatt (1)
  
  1. Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh, 495009, India
  2. School of Biotechnology, Banaras Hindu University, Varanasi, U.P., India
  
• 刊物主题：Biotechnology; Agriculture; Cancer Research; Bioinformatics; Stem Cells; Biomaterials;
• 出版者：Springer Berlin Heidelberg
• ISSN：2190-5738

文摘

An efficient keratinolytic strain of Stenorophomonas maltophilia KB13 was isolated from feather disposal site of Bilaspur, Chhattisgarh, India. The strain could metabolize 10 g/l chicken feathers as sole source of carbon and nitrogen. Soluble protein, amino acid, and cysteine content were found to be maximum (690.6 ± 8.7, 688.9 ± 9.12 and 21 ± 0.36 µg/ml, respectively) at late logarithmic phase of growth. Protease and keratinase activity reached its maximum level (103.26 ± 7.09 and 178.5 ± 9.10 U/ml) at the 4th day of incubation. The feather protein hydrolysate (FPH) obtained after degradation of chicken feathers was utilized to reduce hexavalent chromium. About 78.4 ± 2.4 and 63.6 ± 2.2 % reduction of 50 and 100 mg/l Cr(VI), respectively, was observed after 60 min of incubation with FPH. Further, there was no effect of autoclaved FPH on Cr(VI) reduction indicating that any bacterial enzyme was not involved in reduction process. Cr(VI) reduction was significantly inhibited by 10 mm Hg2+ ions indicating the role of sulfur-containing amino acids in reduction process. FTIR analysis confirmed that chromium reduction occurred due to oxidation of amino acids cysteine and cystine. This study shows that FPH arising after feather degradation can be employed as a potential candidate for the reduction of hexavalant chromium. Keywords Stenotrophomonas maltophilia KB13 Feather degradation Feather protein hydrolysate Cr(VI) reduction