An anterograde rabies virus vector for high-resolution large-scale reconstruction of 3D neuron morphology

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• 作者：Matthias Georg Haberl ; Silvia Viana da Silva…
• 关键词：Neuronal morphology ; Connectivity ; Sparse labeling ; Circuit reconstruction ; Neuron ; type classification ; Alzheimer’s disease
• 刊名：Brain Structure and Function
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：220
• 期：3
• 页码：1369-1379
• 全文大小：4,096 KB
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• 作者单位：Matthias Georg Haberl (1) (2) (3)
  Silvia Viana da Silva (4) (5) (6)
  Jason M. Guest (7)
  Melanie Ginger (1) (2) (4) (5)
  Alexander Ghanem (8)
  Christophe Mulle (4) (5)
  Marcel Oberlaender (10) (7) (9)
  Karl-Klaus Conzelmann (8)
  Andreas Frick (1) (2)
  
  1. Physiopathologie de la plasticité neuronale, INSERM, Neurocentre Magendie, U862, Bordeaux, France
  2. Physiopathologie de la plasticité neuronale, Univ. Bordeaux, Neurocentre Magendie, U862, Bordeaux, France
  3. Institute of NeuroInformatics, University of Zurich, Zurich, Switzerland
  4. Interdisciplinary Institute for Neuroscience, CNRS, UMR 5297, Bordeaux, France
  5. Interdisciplinary Institute for Neuroscience, Univ. Bordeaux, UMR 5297, Bordeaux, France
  6. PDBEB CNC, University of Coimbra, Coimbra, Portugal
  7. Digital Neuroanatomy, Max Planck Florida Institute for Neuroscience, Jupiter, FL, USA
  8. Max-von-Pettenkofer Institute and Gene Center of the Ludwig-Maximilians-University Munich, Munich, Germany
  10. Bernstein Center for Computational Neuroscience, Tuebingen, Germany
  9. Computational Neuroanatomy Group, Max Planck Institute for Biological Cybernetics, Tuebingen, Germany
  
• 刊物主题：Neurosciences; Cell Biology; Neurology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1863-2661

文摘

Glycoprotein-deleted rabies virus (RABV ?G) is a powerful tool for the analysis of neural circuits. Here, we demonstrate the utility of an anterograde RABV ?G variant for novel neuroanatomical approaches involving either bulk or sparse neuronal populations. This technology exploits the unique features of RABV ?G vectors, namely autonomous, rapid high-level expression of transgenes, and limited cytotoxicity. Our vector permits the unambiguous long-range and fine-scale tracing of the entire axonal arbor of individual neurons throughout the brain. Notably, this level of labeling can be achieved following infection with a single viral particle. The vector is effective over a range of ages (>14?months) aiding the studies of neurodegenerative disorders or aging, and infects numerous cell types in all brain regions tested. Lastly, it can also be readily combined with retrograde RABV ?G variants. Together with other modern technologies, this tool provides new possibilities for the investigation of the anatomy and physiology of neural circuits.