Streptomyces zhihengii sp. nov., isolated from rhizospheric soil of Psammosilene tunicoides

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• 作者：Mei-Juan Huang ; Jing-Jing Fei ; Nimaichand Salam ; Chang-Jin Kim…
• 关键词：Streptomyces zhihengii sp. nov. ; Rhizosphere soil ; Lijiang ; Psammosilene tunicoides
• 刊名：Archives of Microbiology
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：198
• 期：8
• 页码：743-749
• 全文大小：475 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Microbial Ecology
  Biochemistry
  Cell Biology
  Biotechnology
  Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-072X
• 卷排序：198

文摘

An actinomycete strain, designated YIM T102T, was isolated from the rhizospheric soil of Psammosilene tunicoides W. C. Wu et C. Y. Wu collected from Lijiang, Yunnan Province, China. The taxonomic position of the new isolate was investigated by a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM T102T belongs to the genus Streptomyces. Strain YIM T102T was most closely related to Streptomyces eurocidicus NRRL B-1676T with a pairwise 16S rRNA gene sequence similarity of 98.9 %. However, DNA–DNA relatedness value between strain YIM T102T and S. eurocidicus NBRC 13491T was found to be 37.8 ± 1.8 %. The menaquinone composition detected for strain YIM T102T was MK-9 (H₆) and MK-9 (H₈), while the major fatty acids were summed feature 4 (38.0 %), anteiso-C_15:0 (13.1 %), iso-C_16:0 (10.1 %), summed feature 3 (9.8 %) and C_16:0 (9.0 %) and iso-C_15:0 (5.2 %). The whole-cell hydrolysates contained galactose, glucose, ribose and mannose, along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 70.7 mol%. Strain YIM T102T also exhibited antagonistic activity against Alternaria alternata, Alternaria brassicae and Colletotrichum nicotianae Averna, based on the findings from the comparative analyses of phenotypic and genotypic characteristics; it is proposed that strain YIM T102 represents a novel species of the genus Streptomyces, for which the name Streptomyces zhihengii sp. nov. is proposed. The type strain is YIM T102T (=KCTC 39115T = DSM 42176T = CGMCC 4.7248T).