In vitro production of haploid and doubled haploid plants from pollinated ovaries of maize (Zea mays)

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• 作者：Feiyu Tang ; Yazhong Tao ; Tianyong Zhao and Guoying Wang
• 关键词：doubled haploid ; haploid ; maize ; ovary culture ; SSR
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2006
• 出版时间：February 2006
• 年：2006
• 卷：84
• 期：2
• 页码：100210-100214
• 全文大小：305 KB

文摘

Haploid induction has potential application for maize breeding. This paper reports that maize haploid plants have been induced by in vitro culture of pollinated ovaries. From a total of 26,400 cultured ovaries, 24 haploid plants were obtained and two of them were doubled after colchicine treatment. The maximum frequency of gynogenesis was 0.17 % at 19.5 h post-pollination (HPP). The results showed that HPP was an important factor affecting plant induction from ovaries. Regenerated diploid R₀ plants were then subjected to genetic analysis using SSR molecular markers. One R₀ plant, whose progeny revealed a high level of homogeneity for several agro-morphological traits, was homozygous at 20 loci tested, with 11 showing paternal and 9 maternal banding pattern. This demonstrates that it is feasible to induce maize haploid plants by in vitro culture of pollinated ovaries.