Multi-elemental bio-imaging of rat tissue from a study investigating the bioavailability of bismuth from shotgun pellets

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• 作者：Dagmar S. Urgast (1)
  Dag G. Ellingsen (2)
  Balázs Berlinger (2)
  Einar Eilertsen (2)
  Grete Friisk (2)
  Vidar Skaug (2)
  Yngvar Thomassen (2)
  John H. Beattie (3)
  In-Sook Kwun (4)
  J?rg Feldmann (1) j.feldmann@abdn.ac.uk
• 关键词：Bismuth – Bio ; imaging – LA ; ICP ; MS – Tissue thin section – Rat
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2012
• 出版时间：July 2012
• 年：2012
• 卷：404
• 期：1
• 页码：89-99
• 全文大小：1.1 MB
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• 作者单位：1. TESLA (Trace Element Speciation Laboratory), University of Aberdeen, College of Physical Sciences, Department of Chemistry, Meston Walk, Aberdeen, AB24 3UE Scotland, UK2. National Institute of Occupational Health, P.O. Box 8149 Dep., 0033 Oslo, Norway3. Rowett Institute of Nutrition and Health, College of Life Sciences and Medicine, Micronutrients Group, University of Aberdeen, Greenburn Road, Bucksburn, Aberdeen, AB21 9SB Scotland, UK4. Department of Food Science and Nutrition, Andong National University, Andong, Kyungpook 760-749, Republic of Korea
• ISSN：1618-2650

文摘

In recent years, bismuth has been promoted as a “green element” and is used as a substitute for the toxic lead in ammunition and other applications. However, the bioavailability and toxicity of bismuth is still not very well described. Following a hunting accident with bismuth-containing shots, a bioavailability study of bismuth from metal pellets inoculated into rat limb muscles was carried out. Bismuth could be found in urine and blood of the animals. Bio-imaging using laser ablation ICP-MS of thin sections of the tissue around the metal implant was carried out to find out more about the distribution of the metal diffusing into the tissue. Two laser ablation systems with different ablation cell designs were compared regarding their analytical performance. Low concentrations of bismuth showing a non-symmetrical pattern were detected in the tissue surrounding the metal implant. This was partly an artefact from cutting the thin sections but also bio-mobilisation of the metals of the implant could be seen. An accumulation of zinc around the implant was interpreted as a marker of inflammation. Challenges regarding sample preparation for laser ablation and bio-imaging of samples of diverse composition became apparent during the analysis.