Felis catus ovary as a model to study follicle biology in vitro

详细信息    查看全文

• 作者：Julieta L. Rojo ; Martina Linari…
• 关键词：Antral follicles ; Felis catus ; Steroids ; Follicle culture ; Cumulus ; oocyte expansion
• 刊名：Journal of Assisted Reproduction and Genetics
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：32
• 期：7
• 页码：1105-1111
• 全文大小：587 KB
• 参考文献：1.Feldman EC, Nelson KW, editors. Canine and feline endocrinology and reproduction. Philadelphia: WB Sounders Co.; 1996.
  2.Wildt DE, Seager SW, Chakraborty PK. Effect of copulatory stimuli on incidence of ovulation and on serum luteinizing hormone in the cat. Endocrinology. 1980;107:1212-.PubMed View Article
  3.Concannon P, Hodgson B, Lein D. Reflex LH release in estrous cats following single and multiple copulations. Biol Reprod. 1980;23:111-.PubMed View Article
  4.Johnson LM, Gay VL. Luteinizing hormone in the cat. II. Mating-induced secretion. Endocrinology. 1981;109:247-2.PubMed View Article
  5.Shille VM, Lundstrom KE, Stabenfeldt GH. Follicular function in the domestic cat as determined by estradiol-17 beta concentrations in plasma: relation to estrous behavior and cornification of exfoliated vaginal epithelium. Biol Reprod. 1979;21:953-3.PubMed View Article
  6.Wildt DE, Comizzoli P, Pukazhenthi B, Songsasen N. Lessons from biodiversity—the value of nontraditional species to advance reproductive science, conservation, and human health. Mol Reprod Dev. 2010;77:397-09.PubMed Central PubMed View Article
  7.Comizzoli P, Wildt DE, Pukazhenthi BS. Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. Mol Reprod Dev. 2008;75:345-4.PubMed Central PubMed View Article
  8.Combelles CM, Cekleniak NA, Racowsky C, Albertini DF. Assessment of nuclear and cytoplasmic maturation in in-vitro matured human oocytes. Hum Reprod. 2002;17:1006-6.PubMed View Article
  9.Silva GM, Rossetto R, Chaves RN, Duarte AB, Araujo VR, Feltrin C, et al. In vitro development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems. Zygote. 2014;26:1-0.
  10.Costa JJ, Passos MJ, Leitao CC, Vasconcelos GL, Saraiva MV, Figueiredo JR, et al. Levels of mRNA for bone morphogenetic proteins, their receptors and SMADs in goat ovarian follicles grown in vivo and in vitro. Reprod Fertil Dev. 2012;24:723-2.PubMed View Article
  11.Valckx SD, Van Hoeck V, Arias-Alvarez M, Maillo V, Lopez-Cardona AP, Gutierrez-Adan A, et al. Elevated non-esterified fatty acid concentrations during in vitro murine follicle growth alter follicular physiology and reduce oocyte developmental competence. Fertil Steril. 2014;102:1769-6.PubMed View Article
  12.Wan X, Zhu Y, Ma X, Zhu J. Establishment of in-vitro mouse preantral follicle culture systems. Wei Sheng Yan Jiu. 2009;38:153-.PubMed
  13.Romero S, Sanchez F, Adriaenssens T, Smitz J. Mouse cumulus-oocyte complexes from in vitro-cultured preantral follicles suggest an anti-luteinizing role for the EGF cascade in the cumulus cells. Biol Reprod. 2011;84:1164-0.PubMed View Article
  14.Xue K, Kim JY, Liu JY, Tsang BK. Insulin-like 3-induced rat preantral follicular growth is mediated by growth differentiation factor 9. Endocrinology. 2014;155:156-7.PubMed View Article
  15.Santos LP, Barros VR, Cavalcante AY, Menezes VG, Macedo TJ, Santos JM, et al. Protein localization of epidermal growth factor in sheep ovaries and improvement of follicle survival and antrum formation in vitro. Reprod Domest Anim. 2014;49:783-.PubMed View Article
  16.Santos JM, Menezes VG, Barberino RS, Macedo TJ, Lins TL, Gouveia BB, et al. Immunohistochemical localization of fibroblast growth factor-2 in the sheep ovary and its effects on pre-antral follicle apoptosis and development in vitro. Reprod Domest Anim. 2014;49:522-.PubMed View Article
  17.Bolamba D, Borden-Russ KD, Durrant BS. In vitro maturation of domestic dog oocytes cultured in advanced preantral and early antral follicles. Theriogenology. 1998;49:933-2.PubMed View Article
  18.Songsasen N, Woodruff TK, Wildt DE. In vitro growth and steroidogenesis of dog follicles are influenced by the physical and hormonal microenvironment. Reproduction. 2011;142:113-2.PubMed Central PubMed View Article
  19.Fujihara M, Comizzoli P, Wildt DE, Songsasen N. Cat and dog primordial follicles enclosed in ovarian cortex sustain viability after in vitro culture on agarose gel in a protein-free medium. Reprod Domest Anim. 2012;47 Suppl 6:102-.PubMed Central PubMed View Article
  20.Xu J, Bernuci MP, Lawson MS, Yeoman RR, Fisher TE, Zelinski MB, et al. Survival, growth, and maturation of secondary follicles from prepubertal, young, and older adult rhesus monkeys during encapsulated three-dimensional culture: effects of gonadotropins and insulin. Reproduction. 2010;140:685-7.PubMed Central PubMed View Article
  21.Xu J, Lawson MS, Yeoman RR, Molskness TA, Ting AY, Stouffer RL, et al. Fibrin promotes development and function of macaque primary follicles during encapsulated three-dimensional culture. Hum Reprod. 2013;28:2187-00.PubMed Central PubMed View Article
  22.Fisher TE, Molskness TA, Villeda A, Zelinski MB, Stouffer RL, Xu J. Vascular endothelial growth factor and angiopoietin produc
• 作者单位：Julieta L. Rojo (1)
  Martina Linari (1)
  Mariana P. Musse (1)
  Marina C. Peluffo (1)
  
  1. Centro de Investigaciones Endocrinológicas “Dr. César Bergadá-(CEDIE), CONICET—FEI—División de Endocrinología, Hospital de Ni?os Ricardo Gutiérrez, Gallo 1330, CABA, Buenos Aires, C1425EFD, Argentina
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Gynecology
  Developmental Biology
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-7330

文摘

Purpose The current study was designed to evaluate the response of individual intact antral follicles from adult female domestic cats to a luteinizing hormone (LH) stimulus in vitro by assessing cumulus-oocyte expansion (C-OE) and steroid production. Methods C-OE and steroid levels (estradiol [E2] and progesterone [P4]) obtained from individual antral feline follicles (n--66 follicles; n--6 cats) were analyzed after 12 or 24?h of culture in the presence or absence of LH (low [3.4?ng/ml] or high [100?ng/ml]). Results At the end of the culture, the highest percentage of expanded cumulus-oocyte complexes (COCs) was observed in the LH groups at 12 or 24?h in comparison to their controls (p-lt;-.001). There was a significant increase in expanded COCs when comparing LH concentrations (high vs. low) at 12 or 24?h. Higher levels of both E2 and P4 were observed in the media from antral follicles after 12 and 24?h of culture in the presence of LH (both concentration, p-lt;-.05). There was no association between hormone levels and follicle diameter; high variability was observed in the steroid levels produced by antral follicles within all treatment groups. Conclusions These data indicate, for the first time, that feline antral follicles (0.5-?mm) from different stages of the natural estrous cycle can be cultured and will respond to an LH stimulus, based on an increase in steroid levels as well as C-OE after 12 or 24?h in culture.