Rhipicephalus (Boophilus) microplus aquaporin as an effective vaccine antigen to protect against cattle tick infestations

详细信息    查看全文

• 作者：Felix D Guerrero (1)
  Renato Andreotti (2)
  Kylie G Bendele (1)
  Rodrigo C Cunha (2)
  Robert J Miller (3)
  Kathleen Yeater (4)
  Adalberto A P茅rez de Le贸n (1)
  
  1. USDA-ARS Knipling Bushland US Livestock Insect Research Laboratory ; 2700 Fredericksburg Rd. ; Kerrville ; TX ; 78028 ; USA
  2. EMBRAPA Beef Cattle ; Avenida Radio Maia ; 830-Vila Popular ; Caixa postal n. 154 ; CEP79106-550 ; Campo Grande ; MS ; Brazil
  3. USDA-ARS Cattle Fever Tick Research Laboratory ; 22675 North Moorefield Rd. ; Edinburg ; TX ; 78541 ; USA
  4. USDA-ARS Southern Plains Area Office ; 1001 Holleman Drive East ; College Station ; TX ; 77845 ; USA
  
• 关键词：Cattle tick ; Recombinant protein ; Vaccine antigen ; Aquaporin
• 刊名：Parasites & Vectors
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：1,860 KB
• 参考文献：1. Grisi L, Leite RC, Martins JRS, Barros ATM, Andreotti R, Cancado PHD, Perez de Leon AA, Pereira JB, Villela HS: Reassessment of the potential economic impact of cattle parasites in Brazil. / Rev Bras Parasitol 2014, In press.
  2. Andreotti, R, Guerrero, FD, Soares, MA, Barros, JC, Miller, RJ, Perez de Leon, A (2011) Rhipicephalus (Boophilus) microplus acaricide resistance in the Brazilian state of Mato Grosso do Sul. Rev Bras Parasitol 20: pp. 127-133 CrossRef
  3. Rodr铆guez-Vivas, RI, Rivas, AL, Chowell, G, Fragoso, SH, Rosario, CR, Garc铆a, Z, Smith, SD, Williams, JJ, Schwager, SJ (2007) Spatial distribution of acaricide profiles (Boophilus microplus strains susceptible or resistant to acaricides) in southeastern Mexico. Vet Parasitol 146: pp. 158-169 j.vetpar.2007.01.016" target="_blank" title="It opens in new window">CrossRef
  4. Guerrero, FD, Leon AA, P, Rodriguez-Vivas, RI, Jonsson, N, Miller, RJ, Andreotti, R Acaricide Research And Development, Resistance And Resistance Monitoring. In: Sonenshine, DE, Roe, RM eds. (2014) Biology of Ticks Volume 2. Oxford University Press, New York, NY, pp. 353-381
  5. Willadsen, P, Bird, P, Cobon, GS, Hungerford, J (1995) Commercialisation of a recombinant vaccine against Boophilus microplus. Parasitol 110: pp. S43-S50 CrossRef
  6. Canales, M, Enriquez, A, Ramos, E, Cabrera, D, Dandie, H, Soto, A, Falcon, V, Rodriguez, M, Fuente, J (1997) Large-scale production in Pichia pastoris of the recombinant vaccine Gavac against cattle tick. Vaccine 15: pp. 414-422 CrossRef
  7. Fuente, J, Almazan, C, Lastra JM, P, Kocan, KM, Willadsen, P (2007) A ten-year review of commercial vaccine performance for control of tick infestations on cattle. Anim Health Res Rev 8: pp. 23-28 CrossRef
  8. Miller, R, Estrada-Pena, A, Almazan, C, Yeater, KM, Messenger, M, Ellis, D, Leon AA, P (2012) Exploring the use of anti-tick vaccine as tool for integrated eradication of the cattle fever tick, Rhipicephalus (Boophilus) annulatus. Vaccine 30: pp. 5682-5687 j.vaccine.2012.05.061" target="_blank" title="It opens in new window">CrossRef
  9. Fragoso, H, Hoshman Rad, P, Ortiz, M, Rodriguez, M, Redondo, M, Herrera, L, Fuente, J (1998) Protection against Boophilus annulatus infestations in cattle vaccinated with the B. microplus Bm86-containing vaccine Gavac. Vaccine 16: pp. 1990-1992 CrossRef
  10. Guerrero, FD, Moolhuijzen, PM, Peterson, DG, Bidwell, S, Caler, E, Appels, R, Bellgard, M, Nene, VM, Djikeng, A (2010) Reassociation kinetics-based approach for partial genome sequencing of the cattle tick, Rhipicephalus (Boophilus) microplus. BMC Genomics 11: pp. 374 CrossRef
  11. Guerrero, FD, Miller, RJ, Rousseau, M-E, Sunkara, S, Quackenbush, J, Lee, Y, Nene, V (2005) BmiGI: a database of cDNAs expressed in Boophilus microplus, the tropical/southern cattle tick. Insect Biochem Mol Biol 35: pp. 585-595 j.ibmb.2005.01.020" target="_blank" title="It opens in new window">CrossRef
  12. Rachinsky, A, Guerrero, FD, Scoles, GA (2007) Differential protein expression in ovaries of uninfected and Babesia-infected southern cattle ticks, Rhipicephalus (Boophilus) microplus. Insect Biochem Mol Biol 37: pp. 1291-1308 j.ibmb.2007.08.001" target="_blank" title="It opens in new window">CrossRef
  13. Rachinsky, A, Guerrero, FD, Scoles, GA (2008) Proteomic profiling of Rhipicephalus (Boophilus) microplus midgut responses to infection with Babesia bovis. Vet Parasitol 152: pp. 294-313 j.vetpar.2007.12.027" target="_blank" title="It opens in new window">CrossRef
  14. Rodriguez-Valle, M, Lew-Tabor, A, Gondro, C, Moolhuijzen, P, Vance, M, Guerrero, FD, Bellgard, M, Jorgensen, W (2010) Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle. BMC Genomics 11: pp. 437 CrossRef
  15. Bellgard, M, Moolhuijzen, PM, Guerrero, FD, Schibeci, D, Rodriguez-Valle, M, Peterson, DG, Dowd, SE, Barrero, R, Hunter, A, Miller, RJ, Lew-Tabor, AE (2012) CattleTickBase: an integrated Internet-based bioinformatics resource for Rhipicephalus (Boophilus) microplus. Int J Parasitol 42: pp. 161-169 j.ijpara.2011.11.006" target="_blank" title="It opens in new window">CrossRef
  16. Rojek, A, Praetorius, J, Frokiaer, J, Nielsen, S, Fenton, RA (2008) A current view of the mammalian aquaglyceroporins. Ann Rev Physiol 70: pp. 301-327 CrossRef
  17. Fu, D, Libson, A, Miercke, LJ, Weitzman, C, Nollert, P, Krucinski, J, Stroud, RM (2000) Structure of a glycerol-conducting channel and the basis for its selectivity. Science 290: pp. 481-486 CrossRef
  18. Denker, BM, Smith, BL, Kuhajda, FP, Agre, P (1988) Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J Biol Chem 263: pp. 15634-15642
  19. Beitz, E, Wu, B, Holm, LM, Schultz, JE, Zeuthen, T (2006) Point mutations in the aromatic/arginine region in aquaporin 1 allow passage of urea, glycerol, ammonia, and protons. Proc Natl Acad Sci U S A 103: pp. 269-274 CrossRef
  20. Megaw, MWJ (1974) Studies on the water balance mechanism of the tick, Boophilus microplus Canestrini. Comp Biochem Physiol 48: pp. 115-125 CrossRef
  21. Saldivar, L, Guerrero, FD, Miller, RJ, Bendele, KG, Gondro, C, Brayton, KA (2008) Microanalysis of acaricide-induced gene expression in the southern cattle tick, Rhipicephalus (Boophilus) microplus. Insect Mol Biol 17: pp. 597-606 j.1365-2583.2008.00831.x" target="_blank" title="It opens in new window">CrossRef
  22. Altschul, SF, Madden, TL, Schaffer, AA, Zhang, J, Zhang, Z, Miller, W, Lipman, DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25: pp. 3389-3402 CrossRef
  23. Altschul, SF, Wootton, JC, Gertz, EM, Agarwala, R, Morgulis, A, Schaffer, AA, Yu, YK (2005) Protein database searches using compositionally adjusted substitution matrices. FEBS J 272: pp. 5101-5109 j.1742-4658.2005.04945.x" target="_blank" title="It opens in new window">CrossRef
  24. Bernsel, A, Viklund, H, Hennerdal, A, Elofsson, A (2009) TOPCONS: consensus prediction of membrane protein topology. Nuc Acids Res 37: pp. W465-W468 CrossRef
  25. Blom, N, Gammeltoft, S, Brunak, S (1999) Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol 294: pp. 1351-1362 jmbi.1999.3310" target="_blank" title="It opens in new window">CrossRef
  26. Bustin, SA, Benes, V, Garson, JA, Hellemands, J, Huggett, J, Kubista, MK, Mueller, R, Nolan, T, Pfaffl, MW, Shipley, GL, Vandesompele, J, Wittwer, CT (2009) The MIQE Guidelines: Minimum Information for publication of Quantitative real-time PCR Experiments. Clin Chem 55: pp. 4 CrossRef
  27. Linder, S, Schliwa, M, Kube-Granderath, E (1996) Direct PCR screening of Pichia pastoris clones. BioTechniques 20: pp. 980-982
  28. Wong, C, Sridhara, S, Bardwell, JCA, Jakob, U (2000) Heating greatly speeds Coomassie Blue staining and destaining. BioTechniques 28: pp. 426-432
  29. Karlsson, M, Fotiadis, D, Sj枚vall, S, Johansson, I, Hedfalk, K, Engel, A, Kjellbom, P (2003) Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastoris. FEBS Lett 537: pp. 68-72 CrossRef
  30. Campbell, EM, Ball, A, Hoppler, S, Bowman, AS (2008) Invertebrate aquaporins: a review. J Comp Physiol B 178: pp. 935-955 CrossRef
  31. Guerrero Jr. F, Perez de Leon AA: Vaccination of animals to elicit a protective immune response against tick infestations and tick-borne pathogen transmission. U. S. Patent 8,722,063, May 13, 2014.
  32. Andreotti, R, Cunha, RC, Soares, MA, Guerrero, FD, Leite, FPL, Perez de Leon, A (2012) Protective immunity against tick infestation in cattle vaccinated with recombinant trypsin inhibitor of Rhipicephalus microplus. Vaccine 30: pp. 6678-6685 j.vaccine.2012.08.066" target="_blank" title="It opens in new window">CrossRef
  33. Campbell, EM, Burdin, M, Hoppler, S, Bowman, AS (2010) Role of aquaporin in the sheep tick Ixodes ricinus: assessment as a potential control target. Int J Parasitol 40: pp. 15-23 j.ijpara.2009.06.010" target="_blank" title="It opens in new window">CrossRef
  34. Ball, A, Campbell, EM, Jacob, J, Hoppler, S, Bowman, AS (2009) Identification, functional characterization and expression patterns of a water-specific aquaporin in the brown dog tick, Rhipicephalus sanguineus. Insect Bioch Mol Biol 39: pp. 105-112 j.ibmb.2008.10.006" target="_blank" title="It opens in new window">CrossRef
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background Vaccination as a control method against the cattle tick, Rhipicephalus (Boophilus) microplus has been practiced since the introduction of two products in the mid-1990s. There is a need for a vaccine that could provide effective control of R. microplus in a more consistent fashion than existing products. During our transcriptome studies of R. microplus, several gene coding regions were discovered to encode proteins with significant amino acid similarity to aquaporins. Methods A cDNA encoding an aquaporin from the cattle tick, Rhipicephalus microplus, was isolated from transcriptomic studies conducted on gut tissues dissected from fully engorged adult female R. microplus. Results Bioinformatic analysis indicates this aquaporin, designated RmAQP1, shows greatest amino acid similarity to the human aquaporin 7 family. Members of this family of water-conducting channels can also facilitate the transport of glycerol in addition to water. The efficacy of this aquaporin as an antigen against the cattle tick was explored in cattle vaccine trials conducted in Brazil. A cDNA encoding a significant portion of RmAQP1 was expressed as a recombinant protein in Pichia pastoris, purified under native conditions using a polyhistidine C-terminus tag and nickel affinity chromatography, emulsified with Montanide adjuvant, and cattle vaccinated intramuscularly. The recombinant protein provided 75% and 68% efficacy in two cattle pen trials conducted in Campo Grande, Brazil on groups of 6 one year old Holstein calves. Conclusion The effectiveness of this vaccine in reducing the numbers of adult female ticks shows this aquaporin antigen holds promise as an active ingredient in cattle vaccines targeted against infestations of R. microplus.