Development of 36 novel polymorphic microsatellites for the critically endangered tree Pinus squamaia, by transcriptome database mining

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• 作者：Jian-Feng Mao (1)
  Bin-Bin Cui (2)
  Zhi-Yong Zhang (3)
  Hui Liu (1)
  Ying-Yue Li (1)
  
  1. National Engineering Laboratory for Tree Breeding ; College of Biological Sciences and Technology ; Beijing Forestry University ; Qinghua East Road No. 35 ; Beijing ; 100083 ; People鈥檚 Republic of China
  2. Department of Biology and Chemistry ; Baoding University ; Baoding ; 071000 ; Hebei ; People鈥檚 Republic of China
  3. National Engineering Research Center for Floriculture ; College of Landscape Architecture ; Beijing Forestry University ; Beijing ; 100083 ; People鈥檚 Republic of China
  
• 关键词：Microstallites ; Pinus squamaia ; Pinus lambertiana ; Transcriptome ; Expressed sequence tag (EST)
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：93-94
• 全文大小：169 KB
• 参考文献：1. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11鈥?5
  2. Kalinowski ST, Taper ML, Marshall TC (2007) Riving how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol 16:1099鈥?106 CrossRef
  3. Peakall R, Smouse PE (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research鈥攁n update. Bioinformatics 28:2537鈥?539 CrossRef
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  5. Zhang ZY, Li DZ (2003) Conservation genetics of an extremely endangered pine, Pinus squamata. Acta Bot Yunnanica 25(5):544鈥?50
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Pinus squamaia X. W. Li is a critically endangered five-needle pine species, with only 35 wild individuals left. To understand its genetic variability, 506 novel microsatellite markers were developed by mining the transcriptome database of P. lambertiana. 196 loci were able to amplify a fragment of expected size in P. squamaia, of which 36 were polymorphic. The number of alleles of these 36 EST-SSRs in 16 individuals varied from 2 to 4, with an average of 2.2. The observed (Ho) and expected (He) heterozygosity values ranged from 0.000 to 1.000 (averaging 0.564) and 0.061 to 0.500 (averaging 0.356), respectively. Twenty-four loci showed significant departure from HWE after Bonferroni correction. This was the first report of candidate molecular markers on P. squamaia, which will facilitate future analyses of genetic diversity and population structure.