Lnc RNA HOTAIR functions as a competing endogenous RNA to regulate HER2 expression by sponging miR-331-3p in gastric cancer

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• 作者：Xiang-hua Liu (1)
  Ming Sun (1)
  Feng-qi Nie (2)
  Ying-bin Ge (3)
  Er-bao Zhang (1)
  Dan-dan Yin (1)
  Rong Kong (1)
  Rui Xia (1)
  Kai-hua Lu (2)
  Jin-hai Li (4)
  Wei De (1)
  Ke-ming Wang (5)
  Zhao-xia Wang (5)
  
  1. Department of Biochemistry and Molecular Biology ; Nanjing Medical University ; Nanjing ; People鈥檚 Republic of China
  2. Department of Oncology ; First Affiliated Hospital ; Nanjing Medical University ; Nanjing ; People鈥檚 Republic of China
  3. Department of Physiology ; Nanjing Medical University ; Nanjing ; People鈥檚 Republic of China
  4. Department of General Surgery ; First Affiliated Hospital ; Nanjing Medical University ; Nanjing ; People鈥檚 Republic of China
  5. Department of Oncology ; Second Affiliated Hospital ; Nanjing Medical University ; Nanjing ; People鈥檚 Republic of China
  
• 关键词：Competing endogenous RNA ; HER2 ; HOTAIR ; Gastric cancer ; Proliferation and invasion
• 刊名：Molecular Cancer
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：13
• 期：1
• 全文大小：2,006 KB
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• 刊物主题：Cancer Research; Oncology;
• 出版者：BioMed Central
• ISSN：1476-4598

文摘

Background Accumulating evidence indicates that the long non-coding RNA HOTAIR plays a critical role in cancer progression and metastasis. However, the overall biological role and clinical significance of HOTAIR in gastric carcinogenesis remains largely unknown. Methods HOTAIR expression was measured in 78 paired cancerous and noncancerous tissue samples by real-time PCR. The effects of HOTAIR on gastric cancer cells were studied by overexpression and RNA interference approaches in vitro and in vivo. Insights of the mechanism of competitive endogenous RNAs (ceRNAs) were gained from bioinformatic analysis, luciferase assays and RNA binding protein immunoprecipitation (RIP). The positive HOTAIR/HER2 interaction was identified and verified by immunohistochemistry assay and bivariate correlation analysis. Results HOTAIR upregulation was associated with larger tumor size, advanced pathological stage and extensive metastasis, and also correlated with shorter overall survival of gastric cancer patients. Furthermore, HOTAIR overexpression promoted the proliferation, migration and invasion of gastric carcinoma cells, while HOTAIR depletion inhibited both cell invasion and cell viability, and induced growth arrest in vitro and in vivo. In particular, HOTAIR may act as a ceRNA, effectively becoming a sink for miR-331-3p, thereby modulating the derepression of HER2 and imposing an additional level of post-transcriptional regulation. Finally, the positive HOTAIR/HER2 correlation was significantly associated with advanced gastric cancers. Conclusions HOTAIR overexpression represents a biomarker of poor prognosis in gastric cancer, and may confer malignant phenotype to tumor cells. The ceRNA regulatory network involving HOTAIR and the positive interaction between HOTAIR and HER2 may contribute to a better understanding of gastric cancer pathogenesis and facilitate the development of lncRNA-directed diagnostics and therapeutics against this disease.