Mapping of the genetic determinant for grain size in rice using a recombinant inbred line (RIL) population generated from two elite indica parents

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• 作者：Dilin Liu ; Meihua Kang ; Feng Wang ; Wuge Liu ; Chongyun Fu ; Jinhua Li…
• 关键词：Rice (Oryza sativa L) ; Grain size ; QTL mapping ; Pleiotropic effect ; Marker ; assisted selection (MAS)
• 刊名：Euphytica
• 出版年：2015
• 出版时间：November 2015
• 年：2015
• 卷：206
• 期：1
• 页码：159-173
• 全文大小：834 KB
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• 作者单位：Dilin Liu (1) (2)
  Meihua Kang (1) (2) (3)
  Feng Wang (1) (2)
  Wuge Liu (1) (2)
  Chongyun Fu (1) (2)
  Jinhua Li (1) (2)
  Manshan Zhu (1) (2)
  Xueqin Zeng (1) (2)
  Yilong Liao (1) (2)
  Zhenrong Liu (1) (2)
  Huijun Huang (1) (2)
  
  1. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China
  2. Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China
  3. Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Physiology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-5060

文摘

Grain size is a major component of rice grain yield and grain quality. Here we conducted genetic analysis for grain size using an F₈ recombinant inbred lines population derived from the cross between two elite indica parents, the slender-grain line Taifeng B with good grain quality and the high-yielding short-grain cultivar Tesanai 2. Strong positive association between hundred-grain weight and grain width was observed, but significant negative correlation was found between grain length and grain width. A total of 36 QTLs for grain weight, grain length, grain width, grain length/width ratio and grain thickness were identified and they explained a total phenotypic variance ranging from 53.6 to 75.2 %. Of them, 6 QTLs were reported for the first time and they are likely novel genetic loci. Moreover, QTLs for grain size tend to form clusters at similar locations. Three of them were detected on chromosome 3, 5, and 7, locating at the regions of the previously reported loci GS3, qSW5/GW5 and GS7, respectively. The allele variance of the above three loci between the parental lines was verified by using functional marker and genome re-sequencing. The major QTLs and QTL clusters discovered in this study are valuable for improvement of rice grain yield and quality through marker-assisted selection. The repeatedly detected QTLs and newly identified loci lay foundation for further fine mapping and map-based cloning. Keywords Rice (Oryza sativa L) Grain size QTL mapping Pleiotropic effect Marker-assisted selection (MAS)