A simple and reliable qualitative detection of six foodstuff powders using optical thin-film biosensor chips

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• 作者：Sulan Bai (1)
  Liang Xu (1)
  Ying Wang (1)
  Xiaolei Yang (1)
  Fengcheng Zhu (1)
  Ying Chen (2)
  Yong Hu (1)
  Fang Bao (1)
  Yikun He (1)
  Yaochuan Zhang (3)
  
• 关键词：Foodstuff flour/powder ; Authentication ; Optical thin ; film biosensor chip
• 刊名：European Food Research and Technology
• 出版年：2013
• 出版时间：May 2013
• 年：2013
• 卷：236
• 期：5
• 页码：899-904
• 全文大小：341KB
• 参考文献：1. Ross M, Jackson L (2012) Summary of the ACS symposium on advances in food allergen detection. J Agric Food Chem. doi:10.1021/jf304327d
  2. Arlorio M, Cereti E, Co茂sson JD, Travaglia F, Martelli FA (2007) Detection of hazelnut ( / Corylus spp.) in processed foods using real-time PCR. Food Control 18:140鈥?48 CrossRef
  3. Hern谩ndez M, Esteve T, Pla M (2005) Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J Agric Food Chem 53:7003鈥?009 CrossRef
  4. Brownie J, Shawcross S, Theaker J, Whitcombe D, Ferrie R, Newton C, Little S (1997) The elimination of primer鈥揹imer accumulation in PCR. Nucleic Acids Res 25:3235鈥?241 CrossRef
  5. B眉ren M, Stadler M, L眉thy J (2001) Detection of wheat adulteration of spelt flour and products by PCR. Eur Food Res Technol 212:234鈥?39 CrossRef
  6. Ding J, Jia J, Yang L, Wen H, Zhang C, Liu W, Zhang D (2004) Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem 52:3372鈥?377 CrossRef
  7. Brodmann PD, Ilg EC, Berthoud H, Herrmann A (2002) Real-time quantitative polymerase chain reaction methods for four genetically modified maize varieties and maize DNA content in food. J AOAC Int 3:85鈥?9
  8. Ramos-G贸mez S, L贸pez-Enr铆quez L, Caminero C, Hern谩ndez M (2008) Pea detection in food and feed samples by a real-time PCR method based on a specific legumin gene that allows diversity analysis. J Agric Food Chem 56:11098鈥?1104 CrossRef
  9. Randhawa GJ, Singh M, Sharma R (2009) Validation of ST-LS1 as an endogenous reference gene for detection of AmA1 and cry1Ab genes in genetically modified potatoes using multiplex and real time PCR. Am J Pot Res 86:398鈥?05 CrossRef
  10. Zhang L, Cao Y, Liu X, Wu G, Wu Y, Lu C (2012) In-depth analysis of the endogenous reference genes used in the quantitative PCR detection systems for rice. Eur Food Res Technol 234:981鈥?93 CrossRef
  11. Hahnen S, Offermann S, Miedl B, R眉ger B, Peterh盲nsel B (2002) Automated DNA preparation from maize tissues and food samples suitable for real-time PCR detection of native genes. Eur Food Res Technol 215:443鈥?46 CrossRef
  12. Mbella E, Lievens A, Barbau-Piednoir E, Sneyers M, Leunda-Casi A, Roosens N, Bulcke M (2011) SYBR^庐Green qPCR methods for detection of endogenous reference genes in commodity crops: a step ahead in combinatory screening of genetically modified crops in food and feed products. Eur Food Res Technol 232:485鈥?96 CrossRef
  13. Handy S, Timme R, Jacob S, Deeds J (2013) Development of a locked nucleic acid real-time polymerase chain reaction assay for the detection of / Pinus armandii in mixed species pine nut samples associated with dysgeusia. J Agric Food Chem. doi:10.1021/jf304223a
  14. Baric S, Kerschbamer C, Dalla VJ (2006) TaqMan real-time PCR versus four conventional PCR assays for detection of apple proliferation phytoplasma. Plant Mol Biol Rep 24:169鈥?84 CrossRef
  15. Bai S, Zhang J, Li S, Zhang X, Chi X, Tian J, Chen H, Luo H, Chen Y, Zhang Y (2010) Detection of six genetically modified maize on optical thin-film biosensor chips. J Agric Food Chem 58:8490鈥?494 CrossRef
  16. Xu L, Bai S, Guo X, Zhang Y, Deng Y, Huang W, Chen Y, He Y (2012) A rapid approach for detecting seven genetically modified canola events using optical thin-film biosensor chips. J Biochips Tissue Chips. doi:10.4172/2153-0777.1000101
  17. Bai S, Li S, Yao T, Hu Y, Bao F, Zhang J, Zhang Y, Zhu S, He Y (2011) Rapid detection of eight vegetable oils on optical thin-film biosensor chips. Food Control 22:1624鈥?628 CrossRef
  18. Bai S, Zhong X, Ma L, Zheng W, Fan L, Wei N, Deng XW (2007) A simple and reliable assay for detecting specific nucleotide sequences in plants using optical thin-film biosensor chips. Plant J 49:354鈥?66 CrossRef
  19. Zhong X, Reynolds R, Kidd JR, Kidd KK, Jenison R, Marlar RA, Ward DC (2003) Single-nucleotide polymorphism genotyping on optical thin-film biosensor chips. Proc Natl Acad Sci 100:11559鈥?1564 CrossRef
  20. Jenison R, Yang S, Haeberli A, Polisky B (2001) Interference-based detection of nucleic acid targets on optically coated silicon. Nat Biotech 19:757鈥?61
  
• 作者单位：Sulan Bai (1)
  Liang Xu (1)
  Ying Wang (1)
  Xiaolei Yang (1)
  Fengcheng Zhu (1)
  Ying Chen (2)
  Yong Hu (1)
  Fang Bao (1)
  Yikun He (1)
  Yaochuan Zhang (3)
  
  1. Key Laboratory of Genetics and Biotechnology, College of Life Sciences, Capital Normal University, Beijing, 100048, People鈥檚 Republic of China
  2. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing, 100123, People鈥檚 Republic of China
  3. Beijing Vocational College of Agriculture, Beijing, 102442, People鈥檚 Republic of China
  
• ISSN：1438-2385

文摘

With the modernization of dietary conceptions, people begin to treat various crops flour as health diet. Unfortunately, species adulteration of flour becomes more and more serious while it is difficult to identify them by the naked eye. Therefore, a reliable molecular biological method of species authentication for common crops flour is required by consumers and authorities. This study developed a reliable and rapid system for detecting six common crops flour (wheat flour, corn flour, potato flour, pea powder, soybean powder and rice flour) using optical silicon thin-film biosensor chips. Aldehyde-labeled capture probes (specific nuclear fragments of endogenous genes of the six food species) were covalently attached to amino-functionalized optical surfaces. Samples containing biotinylated target nucleic acids (PCR amplicon) were then hybridized to this surface. Following hybridization, the immobilized target sequence is incubated with an anti-biotin IgG-horseradish peroxidase conjugate and a precipitable horseradish peroxidase substrate. After incubation, a thin film would be deposited on the chip surface. The mass contributed by the thin film causes a color change visible to the naked human eyes. Using this method, we successfully identified these six food powders. This assay is easy, sensitive, accurate, suitable and can potentially be used as a high throughput for the specific foodstuffs qualitative detection.