Quasi-MSn identification of flavanone 7-glycoside isomers in Da Chengqi Tang by high performance liquid chromatography-tandem mass spectrometry

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• 作者：Fengguo Xu (1) (2)
  Ying Liu (3)
  Zunjian Zhang (1) (2)
  Cheng Yang (1) (2)
  Yuan Tian (1) (2)
  
• 刊名：Chinese Medicine
• 出版年：2009
• 出版时间：December 2009
• 年：2009
• 卷：4
• 期：1
• 全文大小：1605KB
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• 作者单位：Fengguo Xu (1) (2)
  Ying Liu (3)
  Zunjian Zhang (1) (2)
  Cheng Yang (1) (2)
  Yuan Tian (1) (2)
  
  1. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, Jiangsu, 210009, PR China
  2. Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, Jiangsu, 210009, PR China
  3. Department of Pharmacy, Nanjing Municipal Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu, 210001, PR China
  

文摘

Background Da Chengqi Tang (DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from Fructus Aurantii Immaturus. The present study developed an LC-MS/MS method to characterize two pairs of flavanone 7-glycoside isomers, i.e., hesperidin versus neohesperidin and naringin versus isonaringin. Methods After solid phase purification, components in sample were separated on a Agilent zorbax SB-C18 (5 μm, 250 mm × 4.6 mm) analytical column. ESI-MS and quasi-MSn were performed in negative ion mode to obtain structural data of these two pairs of flavanone 7-glycoside isomers. Moreover, UV absorption was measured. Results There was no intra-pairs difference in the UV-Vis and MS/MS spectra of the two pairs of 7-glycoside isomers, whereas the mass spectrometry fragmentation pathways between pairs were different. Conclusion The present study developed a LC-MS/MS method to explore the inter- and intra-pair difference of two pairs of flavanone 7-glycoside isomers.